@inbook{945e394c62354efaac3d31ba9d29cb3c,
title = "High-content RNA interference assay: Analysis of tau hyperphosphorylation as a generic paradigm",
abstract = "High-content analysis methods provide the opportunity to interrogate specific cellular end points in living cells. When coupled with high-throughput RNA interference (ht-RNAi) loss of function screens, high-content analyses are a powerful discovery tool for the identification of new genes and pathways involved in specific disease-relevant cellular functions. The most common readout is a fluorescence measurement, usually based on a green fluorescent protein reporter (or some derivative thereof) or a fluorescently labeled antibody. Here, we describe a specific approach to the development of a high-content assay for the hyperphosphorylation of tau protein that is compatible with RNAi screens. The goal of this chapter is to provide a generic paradigm, using hyperphosphorylation of tau protein as an example, to serve as a blueprint for the investigation of additional cellular end points or protein functions for those interested in performing high-content screens.",
keywords = "Alzheimer's disease, High-content assay, Immunofluorescence, RNA interference, Tau, siRNA",
author = "Robeson, {Rilee H.} and Travis Dunckley",
year = "2011",
month = jan,
day = "1",
doi = "10.1007/978-1-61737-954-3_15",
language = "English (US)",
isbn = "9781617379536",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "221--238",
booktitle = "Disease Gene Identification",
}