Hierarchical Assembly of Nucleic Acid/Coiled-Coil Peptide Nanostructures

Alex Buchberger, Chad R. Simmons, Nour Eddine Fahmi, Ronit Freeman, Nicholas Stephanopoulos

Research output: Contribution to journalArticlepeer-review

61 Scopus citations


DNA and peptides are two of the most commonly used biomolecules for building self-assembling materials, but few examples exist of hybrid nanostructures that contain both components. Here we report the modification of two peptides that comprise a coiled-coil heterodimer pair with unique DNA handles in order to link DNA origami nanostructures bearing complementary strands into micrometer-long one-dimensional arrays. We probed the effect of number of coils on self-assembly and demonstrated the formation of structures through multiple routes: one-pot assembly, formation of dimers and trimers and an alternating copolymer of two different origami structures, and stepwise assembly of purified structures with coiled-coil conjugates. Our results demonstrate the successful merging of two distinct self-assembly modes to create hybrid bionanomaterials expected to have a range of potential applications in the future.

Original languageEnglish (US)
Pages (from-to)1406-1416
Number of pages11
JournalJournal of the American Chemical Society
Issue number3
StatePublished - Jan 22 2020

ASJC Scopus subject areas

  • Catalysis
  • General Chemistry
  • Biochemistry
  • Colloid and Surface Chemistry


Dive into the research topics of 'Hierarchical Assembly of Nucleic Acid/Coiled-Coil Peptide Nanostructures'. Together they form a unique fingerprint.

Cite this