TY - JOUR
T1 - Glomerular Organization in the Antennal Lobe of the Oriental Fruit Fly Bactrocera dorsalis
AU - Lin, Tao
AU - Li, Chaofeng
AU - Liu, Jiali
AU - Smith, Brian
AU - Lei, Hong
AU - Zeng, Xinnian
N1 - Funding Information:
The present research work was financially supported by project (31572314) of the National Natural Science Foundation of China (NSFC), project (2015B090903076) of the Department of Science and Technology of Guangdong Province, and project (GJHZ1140) of the Department of Education of Guangdong Province. The first author is supported by the Doctoral Joint Training Scholarship (2017LHPY022) from South China Agricultural University. The authors are grateful to the Instrument Analysis and Research Center, South China Agricultural University, for the support of the laser scanning confocal microscopy observation.
Publisher Copyright:
© 2018 Lin, Li, Liu, Smith, Lei and Zeng.
PY - 2018/8/29
Y1 - 2018/8/29
N2 - The oriental fruit fly, Bactrocera dorsalis is one of the most destructive pests of horticultural crops in tropical and subtropical Asia. The insect relies heavily on its olfactory system to select suitable hosts for development and reproduction. To understand the neural basis of its odor-driven behaviors, it is fundamental to characterize the anatomy of its olfactory system. In this study, we investigated the anatomical organization of the antennal lobe (AL), the primary olfactory center, in B. dorsalis, and constructed a 3D glomerular atlas of the AL based on synaptic antibody staining combined with computerized 3D reconstruction. To facilitate identification of individual glomeruli, we also applied mass staining of olfactory sensory neurons (OSNs) and projection neurons (PNs). In total, 64 or 65 glomeruli are identifiable in both sexes based on their shape, size, and relative spatial relationship. The overall glomerular volume of two sexes is not statistically different. However, eight glomeruli are sexually dimorphic: four (named AM2, C1, L2, and L3) are larger in males, and four are larger in females (A3, AD1, DM3, and M1). The results from anterograde staining, obtained by applying dye in the antennal lobe, show that three typical medial, media lateral, and lateral antennal-lobe tracts form parallel connections between the antennal lobe and protocerebrum. In addition to these three tracts, we also found a transverse antennal-lobe tract. Based on the retrograde staining of the calyx in the mushroom body, we also characterize the arrangement of roots and cell body clusters linked to the medial antennal-lobe tracts. These data provide a foundation for future studies on the olfactory processing of host odors in B. dorsalis.
AB - The oriental fruit fly, Bactrocera dorsalis is one of the most destructive pests of horticultural crops in tropical and subtropical Asia. The insect relies heavily on its olfactory system to select suitable hosts for development and reproduction. To understand the neural basis of its odor-driven behaviors, it is fundamental to characterize the anatomy of its olfactory system. In this study, we investigated the anatomical organization of the antennal lobe (AL), the primary olfactory center, in B. dorsalis, and constructed a 3D glomerular atlas of the AL based on synaptic antibody staining combined with computerized 3D reconstruction. To facilitate identification of individual glomeruli, we also applied mass staining of olfactory sensory neurons (OSNs) and projection neurons (PNs). In total, 64 or 65 glomeruli are identifiable in both sexes based on their shape, size, and relative spatial relationship. The overall glomerular volume of two sexes is not statistically different. However, eight glomeruli are sexually dimorphic: four (named AM2, C1, L2, and L3) are larger in males, and four are larger in females (A3, AD1, DM3, and M1). The results from anterograde staining, obtained by applying dye in the antennal lobe, show that three typical medial, media lateral, and lateral antennal-lobe tracts form parallel connections between the antennal lobe and protocerebrum. In addition to these three tracts, we also found a transverse antennal-lobe tract. Based on the retrograde staining of the calyx in the mushroom body, we also characterize the arrangement of roots and cell body clusters linked to the medial antennal-lobe tracts. These data provide a foundation for future studies on the olfactory processing of host odors in B. dorsalis.
KW - Antennal lobe
KW - Bactrocera dorsalis
KW - Digital atlas
KW - Glomeruli
KW - Olfaction
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U2 - 10.3389/fnana.2018.00071
DO - 10.3389/fnana.2018.00071
M3 - Article
AN - SCOPUS:85054823861
SN - 1662-5129
VL - 12
JO - Frontiers in Neuroanatomy
JF - Frontiers in Neuroanatomy
M1 - 71
ER -