TY - JOUR
T1 - Genome size and phylogenetic analysis of the A and L races of Botryococcus braunii
AU - Weiss, Taylor L.
AU - Johnston, J. Spencer
AU - Fujisawa, Kazuhiro
AU - Okada, Shigeru
AU - Devarenne, Timothy P.
N1 - Funding Information:
Acknowledgments We would like to thank Dr. Margaret E. Glasner from the Department of Biochemistry and Biophysics at Texas A&M University for help with the phylogenetic analysis. This work was supported by Texas A&M University Department of Biochemistry & Biophysics start-up funds to T.P.D.
PY - 2011/10
Y1 - 2011/10
N2 - Botryococcus braunii (Chlorophyta, Botryococcaceae) is a colony-forming green microalga that produces large amounts of liquid hydrocarbons, which can be converted into transportation fuels. There are three different races of B. braunii, A, B, and L, that are distinguished based on the type of hydrocarbon each produces. Each race also has many strains that are distinguished by the location from which they were collected. While B. braunii has been well studied for the chemistry of the hydrocarbon production, very little is known about the molecular biology of B. braunii. To begin to address this problem, we determined the genome size of the A race, Yamanaka strain, and the L race, Songkla Nakarin strain, of B. braunii. Flow cytometry analysis indicates that the A race of B. braunii has a genome size of 166.0 ± 0.4 Mb, while the L race has a substantially larger genome size at 211.3 ± 1.7 Mb. We also used phylogenetic analysis with the nuclear small subunit (18S) rRNA gene to classify strains of the A and B races that have not yet been compared evolutionarily to previously published B. braunii phylogenetics. The analysis suggests that the evolutionary relationship between B. braunii races is correlated with the type of liquid hydrocarbon they produce.
AB - Botryococcus braunii (Chlorophyta, Botryococcaceae) is a colony-forming green microalga that produces large amounts of liquid hydrocarbons, which can be converted into transportation fuels. There are three different races of B. braunii, A, B, and L, that are distinguished based on the type of hydrocarbon each produces. Each race also has many strains that are distinguished by the location from which they were collected. While B. braunii has been well studied for the chemistry of the hydrocarbon production, very little is known about the molecular biology of B. braunii. To begin to address this problem, we determined the genome size of the A race, Yamanaka strain, and the L race, Songkla Nakarin strain, of B. braunii. Flow cytometry analysis indicates that the A race of B. braunii has a genome size of 166.0 ± 0.4 Mb, while the L race has a substantially larger genome size at 211.3 ± 1.7 Mb. We also used phylogenetic analysis with the nuclear small subunit (18S) rRNA gene to classify strains of the A and B races that have not yet been compared evolutionarily to previously published B. braunii phylogenetics. The analysis suggests that the evolutionary relationship between B. braunii races is correlated with the type of liquid hydrocarbon they produce.
KW - 18S rRNA sequences
KW - Botryococcus braunii
KW - Genome size
KW - Green algae hydrocarbons
KW - Phylogenetics
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U2 - 10.1007/s10811-010-9586-7
DO - 10.1007/s10811-010-9586-7
M3 - Article
AN - SCOPUS:80052660476
SN - 0921-8971
VL - 23
SP - 833
EP - 839
JO - Journal of Applied Phycology
JF - Journal of Applied Phycology
IS - 5
ER -