TY - JOUR
T1 - Genetic evidence that an activation domain of GAL4 does not require acidity and may form a β sheet
AU - Leuther, Kerstin K.
AU - Salmeron, John M.
AU - Johnston, Stephen A.
N1 - Funding Information:
We thank Chris Cotton, Karsten Melcher, Jon Swaffield, Huaqiang Xu, Michael Van Hoy, and Thomas Kodadek for helpful discussions. Jane and David Richardson provided computer facilities, software, helpful suggestions, and comments. We thank Kirsten Fischer Lindahl and Joe Sambrook for critical reading of the manuscript and Steven Trie-zenberg for communicating results prior to publication. This work was supported by National Institutes of Health and Human Frontiers Science Program grants to S. A. J. and by grants from the Perot Family Foundation and the Moss Heart Trust.
PY - 1993/2/26
Y1 - 1993/2/26
N2 - Regulation of gene expression in eukaryotes relies on intricate protein-protein interactions. Transcription of the galactose genes in yeast has been a productive model for this type of interaction. The positive activator in this system, GAL4, has a bifunctional C-terminus. It contains both a prototypic acidic activation domain and a region that binds the negative regulator, GAL80. We have taken advantage of this colocalization of functions to subject the region to a constrained mutagenesis analysis: one function was maintained, while the other one was altered. This analysis and the experiments it suggested have led us to two conclusions: first, the acidic amino acids are not, as commonly thought, required for activation; second, this region is not unstructured or α helical, but its function may require a β sheet.
AB - Regulation of gene expression in eukaryotes relies on intricate protein-protein interactions. Transcription of the galactose genes in yeast has been a productive model for this type of interaction. The positive activator in this system, GAL4, has a bifunctional C-terminus. It contains both a prototypic acidic activation domain and a region that binds the negative regulator, GAL80. We have taken advantage of this colocalization of functions to subject the region to a constrained mutagenesis analysis: one function was maintained, while the other one was altered. This analysis and the experiments it suggested have led us to two conclusions: first, the acidic amino acids are not, as commonly thought, required for activation; second, this region is not unstructured or α helical, but its function may require a β sheet.
UR - http://www.scopus.com/inward/record.url?scp=0027394585&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027394585&partnerID=8YFLogxK
U2 - 10.1016/0092-8674(93)90076-3
DO - 10.1016/0092-8674(93)90076-3
M3 - Article
C2 - 8440021
AN - SCOPUS:0027394585
SN - 0092-8674
VL - 72
SP - 575
EP - 585
JO - Cell
JF - Cell
IS - 4
ER -