TY - JOUR
T1 - Folding analytical devices for electrochemical ELISA in hydrophobic RH paper
AU - Glavan, Ana C.
AU - Christodouleas, Dionysios C.
AU - Mosadegh, Bobak
AU - Yu, Hai Dong
AU - Smith, Barbara S.
AU - Lessing, Joshua
AU - Fernández-Abedul, M. Teresa
AU - Whitesides, George M.
N1 - Publisher Copyright:
© 2014 American Chemical Society.
PY - 2014/12/16
Y1 - 2014/12/16
N2 - This work describes a device for electrochemical enzyme-linked immunosorbent assay (ELISA) designed for low-resource settings and diagnostics at the point of care. The device is fabricated entirely in hydrophobic paper, produced by silanization of paper with decyl trichlorosilane, and comprises two zones separated by a central crease: an embossed microwell, on the surface of which the antigen or antibody immobilization and recognition events occur, and a detection zone where the electrodes are printed. The two zones are brought in contact by folding the device along this central crease; the analytical signal is recorded from the folded configuration. Two proof-of-concept applications, an electrochemical direct ELISA for the detection of rabbit IgG as a model antigen in buffer and an electrochemical sandwich ELISA for the detection of malarial histidine-rich protein from Plasmodium falciparum (Pf HRP2) in spiked human serum, show the versatility of this device. The limit of detection of the electrochemical sandwich ELISA for the quantification of Pf HRP2 in spiked human serum was 4 ng mL-1 (102 pmol L-1), a value within the range of clinically relevant concentrations. (Figure Presented).
AB - This work describes a device for electrochemical enzyme-linked immunosorbent assay (ELISA) designed for low-resource settings and diagnostics at the point of care. The device is fabricated entirely in hydrophobic paper, produced by silanization of paper with decyl trichlorosilane, and comprises two zones separated by a central crease: an embossed microwell, on the surface of which the antigen or antibody immobilization and recognition events occur, and a detection zone where the electrodes are printed. The two zones are brought in contact by folding the device along this central crease; the analytical signal is recorded from the folded configuration. Two proof-of-concept applications, an electrochemical direct ELISA for the detection of rabbit IgG as a model antigen in buffer and an electrochemical sandwich ELISA for the detection of malarial histidine-rich protein from Plasmodium falciparum (Pf HRP2) in spiked human serum, show the versatility of this device. The limit of detection of the electrochemical sandwich ELISA for the quantification of Pf HRP2 in spiked human serum was 4 ng mL-1 (102 pmol L-1), a value within the range of clinically relevant concentrations. (Figure Presented).
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U2 - 10.1021/ac5020782
DO - 10.1021/ac5020782
M3 - Article
C2 - 25470031
AN - SCOPUS:84918576896
SN - 0003-2700
VL - 86
SP - 11999
EP - 12007
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 24
ER -