TY - JOUR
T1 - Fibroblast functionality on novel Ti30Ta nanotube array
AU - Capellato, Patricia
AU - Smith, Barbara S.
AU - Popat, Ketul C.
AU - Claro, Ana P.R.Alves
N1 - Funding Information:
Partial funding support for this work was provided by the Brazilian federal government and the National Council for Scientific and Technological Development (CNPq) via Award Number 201271/2010-9 . The authors would like to thank Patrick McCurdy at Colorado State University for his technical assistance with material characterization.
Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2012/10/1
Y1 - 2012/10/1
N2 - In this study, the mechanical substrate and topographical surface properties of anodized Ti30Ta alloy were investigated using scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS) and contact angle measurement. The anodization process was performed in an electrolyte solution containing HF (48%) and H 2SO 4 (98%) in the volumetric ratios 1:9 with the addition of 5% dimethyl sulfoxide (DMSO) at 15 V, 25 V and 35 V for 20 and 40 min, producing a nanotube architecture when anodized at 35 V for 40 min. Human dermal fibroblasts (HDF, neonatal) were utilized to evaluate the biocompatibility of Ti30Ta nanotubes and Ti30Ta alloy after 1 and 3 days of culture. Cellular adhesion, proliferation, viability, cytoskeletal organization and morphology were investigated using fluorescence microscope imaging, biochemical assay and SEM imaging respectively. The results presented identify altered material properties and improved cellular interaction on Ti30Ta nanotubes as compared to Ti30 Ta alloy.
AB - In this study, the mechanical substrate and topographical surface properties of anodized Ti30Ta alloy were investigated using scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS) and contact angle measurement. The anodization process was performed in an electrolyte solution containing HF (48%) and H 2SO 4 (98%) in the volumetric ratios 1:9 with the addition of 5% dimethyl sulfoxide (DMSO) at 15 V, 25 V and 35 V for 20 and 40 min, producing a nanotube architecture when anodized at 35 V for 40 min. Human dermal fibroblasts (HDF, neonatal) were utilized to evaluate the biocompatibility of Ti30Ta nanotubes and Ti30Ta alloy after 1 and 3 days of culture. Cellular adhesion, proliferation, viability, cytoskeletal organization and morphology were investigated using fluorescence microscope imaging, biochemical assay and SEM imaging respectively. The results presented identify altered material properties and improved cellular interaction on Ti30Ta nanotubes as compared to Ti30 Ta alloy.
KW - Anodization
KW - Human dermal fibroblasts
KW - Ti30Ta nanotubes
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U2 - 10.1016/j.msec.2012.05.013
DO - 10.1016/j.msec.2012.05.013
M3 - Article
AN - SCOPUS:84863305146
SN - 0928-4931
VL - 32
SP - 2060
EP - 2067
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
IS - 7
ER -