Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals:

Salah Awel, Richard Kirian, Max O. Wiedorn, Kenneth R. Beyerlein, Nils Roth, Daniel A. Horke, Dominik Oberthür, Juraj Knoska, Valerio Mariani, Andrew Morgan, Luigi Adriano, Alexandra Tolstikova, P. Lourdu Xavier, Oleksandr Yefanov, Andrew Aquila, Anton Barty, Shatabdi Roy-Chowdhury, Mark S. Hunter, Daniel James, Joseph S. RobinsonUwe Weierstall, Andrei V. Rode, Saša Bajt, Jochen Küpper, Henry N. Chapman

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


High-resolution Bragg diffraction from aerosolized single granulovirus nanocrystals using an X-ray free-electron laser is demonstrated. The outer dimensions of the in-vacuum aerosol injector components are identical to conventional liquid-microjet nozzles used in serial diffraction experiments, which allows the injector to be utilized with standard mountings. As compared with liquid-jet injection, the X-ray scattering background is reduced by several orders of magnitude by the use of helium carrier gas rather than liquid. Such reduction is required for diffraction measurements of small macromolecular nanocrystals and single particles. High particle speeds are achieved, making the approach suitable for use at upcoming high-repetition-rate facilities.

Original languageEnglish (US)
Pages (from-to)133-139
Number of pages7
JournalJournal of Applied Crystallography
Issue number1
StatePublished - Feb 2018


  • X-ray diffraction
  • aerosols
  • nanocrystals

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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