TY - JOUR
T1 - Extensive Wastewater-Based Epidemiology as a Resourceful Tool for SARS-CoV-2 Surveillance in a Low-to-Middle-Income Country through a Successful Collaborative Quest
T2 - WBE, Mobility, and Clinical Tests
AU - Sosa-Hernández, Juan Eduardo
AU - Oyervides-Muñoz, Mariel Araceli
AU - Melchor-Martínez, Elda M.
AU - Driver, Erin M.
AU - Bowes, Devin A.
AU - Kraberger, Simona
AU - Lucero-Saucedo, Sofia Liliana
AU - Fontenele, Rafaela S.
AU - Parra-Arroyo, Lizeth
AU - Holland, Larinda A.
AU - Peña-Benavides, Samantha Ayde
AU - Newell, Melanie Engstrom
AU - Martínez-Ruiz, Manuel
AU - Adhikari, Sangeet
AU - Rodas-Zuluaga, Laura Isabel
AU - Kumar, Rahul
AU - López-Pacheco, Itzel Y.
AU - Castillo-Zacarias, Carlos
AU - Iqbal, Hafiz M.N.
AU - Lim, Efrem S.
AU - Salas-Limón, Daniel
AU - Varsani, Arvind
AU - Halden, Rolf U.
AU - Parra-Saldívar, Roberto
N1 - Funding Information:
Acknowledgments: This project acknowledges the Biotechnology Center—FEMSA and the recently created ad hoc laboratory MARTEC from Tecnológico de Monterey for the development of the project. We also acknowledge the Arizona State University and Biodesign Institute for the fast shift in the previous collaboration supported by the Global Consortium for Sustainability Outcomes with the project title “Facilitating Evidence-based Decision-making for Global Health Out-comes” during the COVID-19 pandemic to increase the capacity for the sample processing to carry out this study. We are thankful to the GIECC Research Group from Tecnológico de Monterey for providing us with the ArcGis Pro license and Duvan Alfonso Alvarado Pachon for the guidance to use the program. CONACYT is thankfully acknowledged for partially supporting this work under the Sistema Nacional de Investigadores (SNI) program awarded to Juan Eduardo Sosa-Hernández (CVU: 375202), Mariel A. Oyervides-Muñoz (CVU: 422778), Elda M. Melchor-Martínez (CVU: 230784), Hafiz M.N. Iqbal (CVU: 735340), Roberto Parra-Saldívar (CVU: 35753), Manuel Martínez-Ruiz (CVU: 418151), and Carlos Castillo-Zacarías (CVU: 359310). Finally, we are thankful to Servicios de Agua y Drenaje de Monterrey for the collaboration in this project.
Funding Information:
We are thankful to Tecnologico de Monterrey for providing financial support to conduct this research. This project acknowledges the Biotechnology Center—FEMSA and the recently created ad hoc laboratory MARTEC from Tecnológico de Monterey for the development of the project. We also acknowledge the Arizona State University and Biodesign Institute for the fast shift in the previous collaboration supported by the Global Consortium for Sustainability Outcomes with the project title “Facilitating Evidence-based Decision-making for Global Health Out-comes” during the COVID-19 pandemic to increase the capacity for the sample processing to carry out this study. We are thankful to the GIECC Research Group from Tecnológico de Monterey for providing us with the ArcGis Pro license and Duvan Alfonso Alvarado Pachon for the guidance to use the program. CONACYT is thankfully acknowledged for partially supporting this work under the Sistema Nacional de Investigadores (SNI) program awarded to Juan Eduardo Sosa-Hernández (CVU: 375202), Mariel A. Oyervides-Muñoz (CVU: 422778), Elda M. Melchor-Martínez (CVU: 230784), Hafiz M.N. Iqbal (CVU: 735340), Roberto Parra-Saldívar (CVU: 35753), Manuel Martínez-Ruiz (CVU: 418151), and Carlos Castillo-Zacarías (CVU: 359310). Finally, we are thankful to Servicios de Agua y Drenaje de Monterrey for the collaboration in this project.
Funding Information:
Funding: We are thankful to Tecnologico de Monterrey for providing financial support to conduct this research.
Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/6/1
Y1 - 2022/6/1
N2 - The COVID-19 pandemic has challenged healthcare systems worldwide. Efforts in low-to-middle-income countries (LMICs) cannot keep stride with infection rates, especially during peaks. A strong international collaboration between Arizona State University (ASU), Tec de Monterrey (TEC), and Servicios de Agua y Drenaje de Monterrey (Local Water Utilities) is acting to integrate wastewater-based epidemiology (WBE) of SARS-CoV-2 in the region as a complementary approach to aid the healthcare system. Wastewater was collected from four sewer catchments in the Monterrey Metropolitan area in Mexico (pop. 4,643,232) from mid-April 2020 to February 2021 (44 weeks, n = 644). Raw wastewater was filtered and filter-concentrated, the RNA was extracted using columns, and the Charité/Berlin protocol was used for the RT-qPCR. The viral loads obtained between the first (June 2020) and second waves (February 2021) of the pandemic were similar; in contrast, the clinical cases were fewer during the first wave, indicating poor coverage. During the second wave of the pandemic, the SARS-CoV-2 quantification in wastewater increased 14 days earlier than the COVID-19 clinical cases reported. This is the first long-term WBE study in Mexico and demonstrates its value in pandemic management.
AB - The COVID-19 pandemic has challenged healthcare systems worldwide. Efforts in low-to-middle-income countries (LMICs) cannot keep stride with infection rates, especially during peaks. A strong international collaboration between Arizona State University (ASU), Tec de Monterrey (TEC), and Servicios de Agua y Drenaje de Monterrey (Local Water Utilities) is acting to integrate wastewater-based epidemiology (WBE) of SARS-CoV-2 in the region as a complementary approach to aid the healthcare system. Wastewater was collected from four sewer catchments in the Monterrey Metropolitan area in Mexico (pop. 4,643,232) from mid-April 2020 to February 2021 (44 weeks, n = 644). Raw wastewater was filtered and filter-concentrated, the RNA was extracted using columns, and the Charité/Berlin protocol was used for the RT-qPCR. The viral loads obtained between the first (June 2020) and second waves (February 2021) of the pandemic were similar; in contrast, the clinical cases were fewer during the first wave, indicating poor coverage. During the second wave of the pandemic, the SARS-CoV-2 quantification in wastewater increased 14 days earlier than the COVID-19 clinical cases reported. This is the first long-term WBE study in Mexico and demonstrates its value in pandemic management.
KW - SARS-CoV-2 in wastewater
KW - SARS-CoV-2 surveillance
KW - public health
KW - wastewater-based epidemiology
UR - http://www.scopus.com/inward/record.url?scp=85132184092&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85132184092&partnerID=8YFLogxK
U2 - 10.3390/w14121842
DO - 10.3390/w14121842
M3 - Article
AN - SCOPUS:85132184092
SN - 2073-4441
VL - 14
JO - Water (Switzerland)
JF - Water (Switzerland)
IS - 12
M1 - 1842
ER -