We operated a sandwich-type microbial electrolysis cell (SMEC) to explore its microbial community under two different electron donors: Acetate and wastewater. We investigated the microbial community using pyrosequencing, quantitative real time PCR (QPCR), and automatic ribosomal intergenic spacer analysis. 454 FLX Titanium pyrosequencing generated 9,752 and 14,428 sequences from anode biofilm samples of the SMEC fed by acetate and wastewater, respectively. We observed higher diversity in anode biofilm from the SMEC fed by wastewater, consisting of several major genera, Trichlorobacter, Desulfomicrobium, Desulfobacter, and Clostridium where the current density was 1.9 A/m 2 (cathodic surface area: 36 cm 2). In contrast, genus Geobacter outcompeted this mixture of microbes and became the most dominant species (63% of total bacteria by QPCR) in the SMEC with acetate, where the current density increased to 38 A/m 2. Several other genera of Pandoraea, Fusibacter, and Acinetobacter appeared as relatively abundant minor players and candidates of anode-respiring bacteria (ARB).

Original languageEnglish (US)
Title of host publicationACS National Meeting Book of Abstracts
StatePublished - 2011
Event241st ACS National Meeting and Exposition - Anaheim, CA, United States
Duration: Mar 27 2011Mar 31 2011


Other241st ACS National Meeting and Exposition
Country/TerritoryUnited States
CityAnaheim, CA

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)


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