Evolutionary conservation and molecular cloning of the recombinase activating gene 1++

Ralph M. Bernstein, Samuel F. Schluter, Douglas F. Lake, John J. Marchalonis

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


A 700-bp fragment of the recombinase activating gene 1 (RAG-1) was cloned from several evolutionarily distant (sandbar shark, paddlefish, goldfish, axolotl and pig) species using PCR. The nucleotide and deduced amino acid sequences revealed a highly conserved region that has remained essentially unaltered during 400 million years of evolution; e.g, shark and human sequences were 75% identical at the nucleic acid level and 87% as protein. The RAG-1 mRNA levels in the shark were analyzed using semi-quantitative PCR to reveal expression patterns contrary to normal mammalian expression. These results establish that the genetic mechisms for Ig gene rearrangement are present in all extant gnathanstomes.

Original languageEnglish (US)
Pages (from-to)687-692
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number1
StatePublished - Nov 30 1994
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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