TY - JOUR
T1 - Evolutionary conservation and molecular cloning of the recombinase activating gene 1++
AU - Bernstein, Ralph M.
AU - Schluter, Samuel F.
AU - Lake, Douglas F.
AU - Marchalonis, John J.
PY - 1994/11/30
Y1 - 1994/11/30
N2 - A 700-bp fragment of the recombinase activating gene 1 (RAG-1) was cloned from several evolutionarily distant (sandbar shark, paddlefish, goldfish, axolotl and pig) species using PCR. The nucleotide and deduced amino acid sequences revealed a highly conserved region that has remained essentially unaltered during 400 million years of evolution; e.g, shark and human sequences were 75% identical at the nucleic acid level and 87% as protein. The RAG-1 mRNA levels in the shark were analyzed using semi-quantitative PCR to reveal expression patterns contrary to normal mammalian expression. These results establish that the genetic mechisms for Ig gene rearrangement are present in all extant gnathanstomes.
AB - A 700-bp fragment of the recombinase activating gene 1 (RAG-1) was cloned from several evolutionarily distant (sandbar shark, paddlefish, goldfish, axolotl and pig) species using PCR. The nucleotide and deduced amino acid sequences revealed a highly conserved region that has remained essentially unaltered during 400 million years of evolution; e.g, shark and human sequences were 75% identical at the nucleic acid level and 87% as protein. The RAG-1 mRNA levels in the shark were analyzed using semi-quantitative PCR to reveal expression patterns contrary to normal mammalian expression. These results establish that the genetic mechisms for Ig gene rearrangement are present in all extant gnathanstomes.
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U2 - 10.1006/bbrc.1994.2720
DO - 10.1006/bbrc.1994.2720
M3 - Article
C2 - 7999099
AN - SCOPUS:0027985076
SN - 0006-291X
VL - 205
SP - 687
EP - 692
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -