Abstract
Yersinia pestis PsaA is an adhesin that is synthesized inside macrophages. Here, we evaluated the immune profile of codon-optimized Y. pestis PsaA synthesized in a live recombinant attenuated Salmonella vaccine (RASV) strain χ9558. Oral immunization of BALB/c mice with χ9558(pYA3705) delivering a secreted form of PsaA, elicited a systemic PsaA-specific immunoglobulin G (IgG) response but offered limited protection against lethal challenge with the intranasally introduced Y. pestis CO92 strain. Our results suggest that appropriate fine-tuning of Y. pestis PsaA delivery by RASV could improve its protective role in curtailing plague colonization and infection.
Original language | English (US) |
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Pages (from-to) | 5810-5816 |
Number of pages | 7 |
Journal | Vaccine |
Volume | 28 |
Issue number | 36 |
DOIs | |
State | Published - Aug 2010 |
Keywords
- Asd
- Codon-optimized
- PsaA antigen
- PsaB chaperone protein
- PsaC usher protein
- Vaccine plasmid
ASJC Scopus subject areas
- Molecular Medicine
- General Immunology and Microbiology
- General Veterinary
- Public Health, Environmental and Occupational Health
- Infectious Diseases