Evaluating the effect of spaceflight on the host–pathogen interaction between human intestinal epithelial cells and Salmonella Typhimurium

Jennifer Barrila; Shameema F. Sarker; Nicole Hansmeier; Shanshan Yang; Kristina Buss; Natalia Briones; Jin Park; Richard R. Davis; Rebecca J. Forsyth; C. Mark Ott; Kevin Sato; Cristine Kosnik; Anthony Yang; Cheryl Shimoda; Nicole Rayl; Diana Ly; Aaron Landenberger; Stephanie D. Wilson; Naoko Yamazaki; Jason Steel; Camila Montano; Rolf U. Halden; Tom Cannon; Sarah L. Castro-Wallace; Cheryl A. Nickerson

doi:10.1038/s41526-021-00136-w

Evaluating the effect of spaceflight on the host–pathogen interaction between human intestinal epithelial cells and Salmonella Typhimurium

Jennifer Barrila, Shameema F. Sarker, Nicole Hansmeier, Shanshan Yang, Kristina Buss, Natalia Briones, Jin Park, Richard R. Davis, Rebecca J. Forsyth, C. Mark Ott, Kevin Sato, Cristine Kosnik, Anthony Yang, Cheryl Shimoda, Nicole Rayl, Diana Ly, Aaron Landenberger, Stephanie D. Wilson, Naoko Yamazaki, Jason SteelCamila Montano, Rolf U. Halden, Tom Cannon, Sarah L. Castro-Wallace, Cheryl A. Nickerson

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Spaceflight uniquely alters the physiology of both human cells and microbial pathogens, stimulating cellular and molecular changes directly relevant to infectious disease. However, the influence of this environment on host–pathogen interactions remains poorly understood. Here we report our results from the STL-IMMUNE study flown aboard Space Shuttle mission STS-131, which investigated multi-omic responses (transcriptomic, proteomic) of human intestinal epithelial cells to infection with Salmonella Typhimurium when both host and pathogen were simultaneously exposed to spaceflight. To our knowledge, this was the first in-flight infection and dual RNA-seq analysis using human cells.

Original language	English (US)
Article number	9
Journal	npj Microgravity
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/s41526-021-00136-w
State	Published - Dec 2021

ASJC Scopus subject areas

Medicine (miscellaneous)
Materials Science (miscellaneous)
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Agricultural and Biological Sciences (miscellaneous)
Physics and Astronomy (miscellaneous)
Space and Planetary Science

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10.1038/s41526-021-00136-w

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Barrila, J., Sarker, S. F., Hansmeier, N., Yang, S., Buss, K., Briones, N., Park, J., Davis, R. R., Forsyth, R. J., Ott, C. M., Sato, K., Kosnik, C., Yang, A., Shimoda, C., Rayl, N., Ly, D., Landenberger, A., Wilson, S. D., Yamazaki, N., ... Nickerson, C. A. (2021). Evaluating the effect of spaceflight on the host–pathogen interaction between human intestinal epithelial cells and Salmonella Typhimurium. npj Microgravity, 7(1), Article 9. https://doi.org/10.1038/s41526-021-00136-w

Barrila, J, Sarker, SF, Hansmeier, N, Yang, S, Buss, K, Briones, N, Park, J, Davis, RR, Forsyth, RJ, Ott, CM, Sato, K, Kosnik, C, Yang, A, Shimoda, C, Rayl, N, Ly, D, Landenberger, A, Wilson, SD, Yamazaki, N, Steel, J, Montano, C, Halden, RU, Cannon, T, Castro-Wallace, SL & Nickerson, CA 2021, 'Evaluating the effect of spaceflight on the host–pathogen interaction between human intestinal epithelial cells and Salmonella Typhimurium', npj Microgravity, vol. 7, no. 1, 9. https://doi.org/10.1038/s41526-021-00136-w

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abstract = "Spaceflight uniquely alters the physiology of both human cells and microbial pathogens, stimulating cellular and molecular changes directly relevant to infectious disease. However, the influence of this environment on host–pathogen interactions remains poorly understood. Here we report our results from the STL-IMMUNE study flown aboard Space Shuttle mission STS-131, which investigated multi-omic responses (transcriptomic, proteomic) of human intestinal epithelial cells to infection with Salmonella Typhimurium when both host and pathogen were simultaneously exposed to spaceflight. To our knowledge, this was the first in-flight infection and dual RNA-seq analysis using human cells.",

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AU - Yang, Shanshan

AU - Buss, Kristina

AU - Briones, Natalia

AU - Park, Jin

AU - Davis, Richard R.

AU - Forsyth, Rebecca J.

AU - Ott, C. Mark

AU - Sato, Kevin

AU - Kosnik, Cristine

AU - Yang, Anthony

AU - Shimoda, Cheryl

AU - Rayl, Nicole

AU - Ly, Diana

AU - Landenberger, Aaron

AU - Wilson, Stephanie D.

AU - Yamazaki, Naoko

AU - Steel, Jason

AU - Montano, Camila

AU - Halden, Rolf U.

AU - Cannon, Tom

AU - Castro-Wallace, Sarah L.

AU - Nickerson, Cheryl A.

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Evaluating the effect of spaceflight on the host–pathogen interaction between human intestinal epithelial cells and Salmonella Typhimurium

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