TY - JOUR
T1 - Electro-selective fermentation enhances lipid extraction and biohydrogenation of Scenedesmus acutus biomass
AU - Liu, Yuanzhe
AU - Lai, Yen Jung Sean
AU - Barbosa, Thiago Stangherlin
AU - Chandra, Rashmi
AU - Parameswaran, Prathap
AU - Rittmann, Bruce E.
N1 - Funding Information:
This project is supported by the U.S. National Scientific Foundation (award number 1509933 ). We thank Dr. John McGowen at the Arizona Center of Algal Technology and Innovation (AzCATI) for providing the algal biomass, Dr. Everett Eustance for his excellence at maintaining Photo-Bio Reactors, Mr. David Lowry at the Electron microscopy facility of the School of Life Sciences at Arizona State University for his expertise at Transmission Electronic Microscopy, and Dr. Juan Maldonado Ortiz at the Center for Microbiome Sequencing for his expertise in gene amplification and sequencing.
Publisher Copyright:
© 2019 Elsevier B.V.
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2019/3
Y1 - 2019/3
N2 - Extraction of lipids from microalgae has been economically and energy prohibitive due to the requirement for pretreatment, and the use of toxic solvents in the process has exacerbated the problem. Selective Fermentation (SF) helps overcome these problems by selectively biodegrading carbohydrate and protein, while conserving lipids. We introduced and evaluated electro-selective fermentation (ESF), in which anode respiration in a microbial electrolysis cell (MEC) altered fermentation performance in ways that enhanced lipid wet-extraction. Using Scenedesmus acutus biomass, we evaluated ESF and compared it to SF. Compared to SF, ESF increased protein degradation by 4-fold, even though anode respiration represented <1% of the total electron input. Although ESF led to greater loss of total lipids, it improved lipid wet-extraction efficiency using a non-toxic solvent by 3-fold. Lipid loss was due to β-oxidation linked to biohydrogenation, and the long-chain fatty acid (LCFA) profile shifted from C18:1 to C16:0 and C14:0. Microbial community analysis emphasized the presence of protein-degrading bacteria and biohydrogenators in the ESF suspension and anode-respiring bacteria (ARB) on the ESF anode. Overall, ESF improved lipid extractability and biofuel quality despite current being a small component in the overall COD balance.
AB - Extraction of lipids from microalgae has been economically and energy prohibitive due to the requirement for pretreatment, and the use of toxic solvents in the process has exacerbated the problem. Selective Fermentation (SF) helps overcome these problems by selectively biodegrading carbohydrate and protein, while conserving lipids. We introduced and evaluated electro-selective fermentation (ESF), in which anode respiration in a microbial electrolysis cell (MEC) altered fermentation performance in ways that enhanced lipid wet-extraction. Using Scenedesmus acutus biomass, we evaluated ESF and compared it to SF. Compared to SF, ESF increased protein degradation by 4-fold, even though anode respiration represented <1% of the total electron input. Although ESF led to greater loss of total lipids, it improved lipid wet-extraction efficiency using a non-toxic solvent by 3-fold. Lipid loss was due to β-oxidation linked to biohydrogenation, and the long-chain fatty acid (LCFA) profile shifted from C18:1 to C16:0 and C14:0. Microbial community analysis emphasized the presence of protein-degrading bacteria and biohydrogenators in the ESF suspension and anode-respiring bacteria (ARB) on the ESF anode. Overall, ESF improved lipid extractability and biofuel quality despite current being a small component in the overall COD balance.
KW - Biohydrogenation
KW - Electro-selective fermentation (ESF)
KW - Lipids
KW - Long-chain fatty acids (LCFA)
KW - Microbial electrolysis cells (MEC)
KW - Scenedesmus
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U2 - 10.1016/j.algal.2018.101397
DO - 10.1016/j.algal.2018.101397
M3 - Article
AN - SCOPUS:85059744318
SN - 2211-9264
VL - 38
JO - Algal Research
JF - Algal Research
M1 - 101397
ER -