TY - JOUR
T1 - Effect of phenobarbital administration to rats on the level of the in vitro synthesis of cytochrome P-450 directed by total rat liver RNA
AU - Dubois, Raymond N.
AU - Waterman, Michael R.
N1 - Funding Information:
The authors thank Dr. A.Y.H. Lu and Susan West of the Merck, Sharp and Dohme Research Laboratories for their generous gift of the purified cytochrome P-450. We appreciate the encouragement and advice of Dr. R.W. Estabrook and Dr. R.A. Butow and thank Dr. J. Werringloer for helping in the spectrophotometric determinations of cytochrome P-450. Finally, we acknowledge Pamela Pickard for technical assistance and Lisa Abrams for art work. This research was supported by Grant No. I-624 from The Robert A. Welch Foundation and NIGMS 16488 from the U.S. Public Health Service.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1979/9/12
Y1 - 1979/9/12
N2 - Total liver RNA has been isolated from male rats at different time points subsequent to a single injection of phenobarbital, and the level of cytochrome P-450 synthesis directed by these RNA preparations in a cell-free translation system has been determined. It is observed that the maximum in vitro synthesis of cytochrome P-450 occurs at 16 hours (3-fold above uninduced level) which is approximately 30 hours prior to the maximum induction of spectrophotometrically detectable cytochrome P-450 measured in liver homogenates. Thus, while cytochrome P-450 mRNA is involved in the induction process, its synthesis does not appear to be rate limiting. In addition, phenobarbital induced cytochrome P-450 is not synthesized in vitro in a form larger than that isolated from endoplasmic reticulum, but rather is also found to have a molecular weight of 50,000.
AB - Total liver RNA has been isolated from male rats at different time points subsequent to a single injection of phenobarbital, and the level of cytochrome P-450 synthesis directed by these RNA preparations in a cell-free translation system has been determined. It is observed that the maximum in vitro synthesis of cytochrome P-450 occurs at 16 hours (3-fold above uninduced level) which is approximately 30 hours prior to the maximum induction of spectrophotometrically detectable cytochrome P-450 measured in liver homogenates. Thus, while cytochrome P-450 mRNA is involved in the induction process, its synthesis does not appear to be rate limiting. In addition, phenobarbital induced cytochrome P-450 is not synthesized in vitro in a form larger than that isolated from endoplasmic reticulum, but rather is also found to have a molecular weight of 50,000.
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U2 - 10.1016/0006-291X(79)91602-4
DO - 10.1016/0006-291X(79)91602-4
M3 - Article
C2 - 496967
AN - SCOPUS:0018725024
SN - 0006-291X
VL - 90
SP - 150
EP - 157
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -