TY - GEN
T1 - Droplet triggering for serial femtosecond crystallography using 3d-printed microfluidics
AU - Kim, Daihyun
AU - Echelmeier, Austin
AU - Villarreal, Jorvani Cruz
AU - Gandhi, Sahir
AU - Quintana, Sebastian
AU - Egatz-Gomez, Ana
AU - Ros, Alexandra
N1 - Funding Information:
We greatly acknowledge Dr. Kirian and Dr. Weierstall at the Physics Department at Arizona State University for support with the high-speed camera.
Publisher Copyright:
© 2019 CBMS-0001.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2019
Y1 - 2019
N2 - Structural biology at X-ray free-electron lasers (XFELs) is a compelling new technique. The majority of protein crystal sample, however, is wasted when continuously injected into the XFEL beam. Here, we present a new advancement in microfluidics by delivering crystal sample in droplets intersected by an oil phase with electrical control over the droplet generation parameters. Using integrated electrodes in a 3D-printed device, we demonstrate droplet generation, control of droplet frequency, and introduction of a phase shift for synchronization with the XFEL. This method has the potential to reduce sample waste for structural biology with XFELs by > 95%.
AB - Structural biology at X-ray free-electron lasers (XFELs) is a compelling new technique. The majority of protein crystal sample, however, is wasted when continuously injected into the XFEL beam. Here, we present a new advancement in microfluidics by delivering crystal sample in droplets intersected by an oil phase with electrical control over the droplet generation parameters. Using integrated electrodes in a 3D-printed device, we demonstrate droplet generation, control of droplet frequency, and introduction of a phase shift for synchronization with the XFEL. This method has the potential to reduce sample waste for structural biology with XFELs by > 95%.
KW - 3D-printing
KW - Droplet microfluidics
KW - Electrowetting
KW - Serial Femtosecond Crystallography
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M3 - Conference contribution
AN - SCOPUS:85094942054
T3 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
SP - 1470
EP - 1471
BT - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PB - Chemical and Biological Microsystems Society
T2 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Y2 - 27 October 2019 through 31 October 2019
ER -