DNA Sequencing-by-Synthesis using Novel Nucleotide Analogs

Lin Yu, Jia Guo, Ning Xu, Zengmin Li, Jingyue Ju

Research output: Chapter in Book/Report/Conference proceedingChapter


The completion of the Human Genome Project has increased the need for highthroughput DNA sequencing technologies aimed at uncovering the genomic contributions to diseases. The DNA sequencing-by-synthesis (SBS) approach has shown great promise as a new platform for deciphering the genome. Recently, much progress has been made in the fundamental sciences required to make SBS a viable sequencing technology. One of the unique features of this approach is that many of the steps required are compatible in a modular fashion, allowing for the best solution at each stage to be effectively integrated. Recent advances include the design and synthesis of novel cleavable fluorescent nucleotide reversible terminators, DNA template preparation using emulsion polymerase chain reaction and clonal clusters on immobilized single DNA molecules, and new surface attachment chemistries for DNA template immobilization. The integration of these advances will lead to the development of a high-throughputDNAsequencing system based on SBS that is able to decipher an entire human genome for $1000 in the near future.

Original languageEnglish (US)
Title of host publicationThe Handbook of Plant Mutation Screening
Subtitle of host publicationMining of Natural and Induced Alleles
Number of pages20
ISBN (Print)9783527326044
StatePublished - Mar 30 2010
Externally publishedYes


  • Fluorescence
  • Four-color DNA sequencing
  • Hybrid DNA sequencing
  • Novel nucleotide analog
  • Nucleotide reversible terminator
  • Sequencing-by-hybridization

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology


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