DNA polymerase-mediated DNA synthesis on a TNA template

John C. Chaput, Justin K. Ichida, Jack W. Szostak

Research output: Contribution to journalArticlepeer-review

96 Scopus citations


TNA, or threose nucleic acid, is capable of Watson-Crick base pairing with DNA, RNA, and TNA; coupled with its chemical simplicity, this suggests that TNA is a possible progenitor of RNA. As an initial step toward developing the molecular tools necessary to investigate the functional capabilities of TNA by in vitro selection, we have screened a variety of DNA polymerases for activity on a TNA template. We report that despite having a repeating unit that is one atom shorter than that of DNA, several polymerases showed surprisingly good ability to copy limited stretches of TNA.

Original languageEnglish (US)
Pages (from-to)856-857
Number of pages2
JournalJournal of the American Chemical Society
Issue number4
StatePublished - Jan 29 2003
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry


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