DNA polymerase-mediated DNA synthesis on a TNA template

John C. Chaput; Justin K. Ichida; Jack W. Szostak

doi:10.1021/ja028589k

DNA polymerase-mediated DNA synthesis on a TNA template

John C. Chaput, Justin K. Ichida, Jack W. Szostak

Research output: Contribution to journal › Article › peer-review

96 Scopus citations

Abstract

TNA, or threose nucleic acid, is capable of Watson-Crick base pairing with DNA, RNA, and TNA; coupled with its chemical simplicity, this suggests that TNA is a possible progenitor of RNA. As an initial step toward developing the molecular tools necessary to investigate the functional capabilities of TNA by in vitro selection, we have screened a variety of DNA polymerases for activity on a TNA template. We report that despite having a repeating unit that is one atom shorter than that of DNA, several polymerases showed surprisingly good ability to copy limited stretches of TNA.

Original language	English (US)
Pages (from-to)	856-857
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	125
Issue number	4
DOIs	https://doi.org/10.1021/ja028589k
State	Published - Jan 29 2003
Externally published	Yes

ASJC Scopus subject areas

Catalysis
Chemistry(all)
Biochemistry
Colloid and Surface Chemistry

Access to Document

10.1021/ja028589k

Cite this

@article{a01538e744dd452892108312f5c2b2c7,

title = "DNA polymerase-mediated DNA synthesis on a TNA template",

abstract = "TNA, or threose nucleic acid, is capable of Watson-Crick base pairing with DNA, RNA, and TNA; coupled with its chemical simplicity, this suggests that TNA is a possible progenitor of RNA. As an initial step toward developing the molecular tools necessary to investigate the functional capabilities of TNA by in vitro selection, we have screened a variety of DNA polymerases for activity on a TNA template. We report that despite having a repeating unit that is one atom shorter than that of DNA, several polymerases showed surprisingly good ability to copy limited stretches of TNA.",

author = "Chaput, {John C.} and Ichida, {Justin K.} and Szostak, {Jack W.}",

year = "2003",

month = jan,

day = "29",

doi = "10.1021/ja028589k",

language = "English (US)",

volume = "125",

pages = "856--857",

journal = "Journal of the American Chemical Society",

issn = "0002-7863",

publisher = "American Chemical Society",

number = "4",

}

TY - JOUR

T1 - DNA polymerase-mediated DNA synthesis on a TNA template

AU - Chaput, John C.

AU - Ichida, Justin K.

AU - Szostak, Jack W.

PY - 2003/1/29

Y1 - 2003/1/29

N2 - TNA, or threose nucleic acid, is capable of Watson-Crick base pairing with DNA, RNA, and TNA; coupled with its chemical simplicity, this suggests that TNA is a possible progenitor of RNA. As an initial step toward developing the molecular tools necessary to investigate the functional capabilities of TNA by in vitro selection, we have screened a variety of DNA polymerases for activity on a TNA template. We report that despite having a repeating unit that is one atom shorter than that of DNA, several polymerases showed surprisingly good ability to copy limited stretches of TNA.

AB - TNA, or threose nucleic acid, is capable of Watson-Crick base pairing with DNA, RNA, and TNA; coupled with its chemical simplicity, this suggests that TNA is a possible progenitor of RNA. As an initial step toward developing the molecular tools necessary to investigate the functional capabilities of TNA by in vitro selection, we have screened a variety of DNA polymerases for activity on a TNA template. We report that despite having a repeating unit that is one atom shorter than that of DNA, several polymerases showed surprisingly good ability to copy limited stretches of TNA.

UR - http://www.scopus.com/inward/record.url?scp=0037471633&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037471633&partnerID=8YFLogxK

U2 - 10.1021/ja028589k

DO - 10.1021/ja028589k

M3 - Article

C2 - 12537469

AN - SCOPUS:0037471633

SN - 0002-7863

VL - 125

SP - 856

EP - 857

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 4

ER -

DNA polymerase-mediated DNA synthesis on a TNA template

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this