Dissecting the regulation of yeast genes by the osmotin receptor

Brian R. Kupchak, Nancy Y. Villa, Lidia V. Kulemina, Thomas J. Lyons

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


The Izh2p protein from Saccharomyces cerevisiae is a receptor for the plant antifungal protein, osmotin. Since Izh2p is conserved in fungi, understanding its biochemical function could inspire novel strategies for the prevention of fungal growth. However, it has been difficult to determine the exact role of Izh2p because it has pleiotropic effects on cellular biochemistry. Herein, we demonstrate that Izh2p negatively regulates functionally divergent genes through a CCCTC promoter motif. Moreover, we show that Izh2p-dependent promoters containing this motif are regulated by the Nrg1p/Nrg2p and Msn2p/Msn4p transcription factors. The fact that Izh2p can regulate gene expression through this widely dispersed element presents a reasonable explanation of its pleiotropy. The involvement of Nrg1p/Nrgp2 in Izh2p-dependent gene regulation also suggests a role for this receptor in regulating fungal differentiation in response to stimuli produced by plants.

Original languageEnglish (US)
Pages (from-to)210-213
Number of pages4
JournalBiochemical and Biophysical Research Communications
Issue number2
StatePublished - Sep 19 2008


  • IZH2
  • Iron
  • Izh2p
  • Osmotin
  • Yeast
  • Zinc

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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