TY - JOUR
T1 - Differential modulation of E. coli mRNA abundance by inhibitory proteins that alter the composition of the degradosome
AU - Gao, Junjun
AU - Lee, Kangseok
AU - Zhao, Meng
AU - Qiu, Ji
AU - Zhan, Xiaoming
AU - Saxena, Ankur
AU - Moore, Christopher J.
AU - Cohen, Stanley N.
AU - Georgiou, George
PY - 2006/7
Y1 - 2006/7
N2 - In Escherichia coli the initial step in the processing or decay of many messenger and structural RNAs is mediated by the endonuclease RNase E, which forms the core of a large RNA-catalysis machine termed the degradosome. Previous experiments have identified a protein that globally modulates RNA abundance by binding to RNase E and regulating its endonucleolytic activity. Here we report the discovery of RraB, which interacts with a different site on RNase E and interferes with cleavage of a different set of transcripts. We show that expression of RraA or RraB in vivo is accompanied by dramatic, distinct, and inhibitor-specific changes in degradosome composition - and that these are in turn associated with alterations in RNA decay and global transcript abundance profiles that are dissimilar to the profile observed during simple RNase E deficiency. Our results reveal the existence of endonuclease binding proteins that modulate the remodelling of degradosome composition in bacteria and argue that such degradosome remodelling is a mechanism for the differential regulation of RNA cleavages in E. coli.
AB - In Escherichia coli the initial step in the processing or decay of many messenger and structural RNAs is mediated by the endonuclease RNase E, which forms the core of a large RNA-catalysis machine termed the degradosome. Previous experiments have identified a protein that globally modulates RNA abundance by binding to RNase E and regulating its endonucleolytic activity. Here we report the discovery of RraB, which interacts with a different site on RNase E and interferes with cleavage of a different set of transcripts. We show that expression of RraA or RraB in vivo is accompanied by dramatic, distinct, and inhibitor-specific changes in degradosome composition - and that these are in turn associated with alterations in RNA decay and global transcript abundance profiles that are dissimilar to the profile observed during simple RNase E deficiency. Our results reveal the existence of endonuclease binding proteins that modulate the remodelling of degradosome composition in bacteria and argue that such degradosome remodelling is a mechanism for the differential regulation of RNA cleavages in E. coli.
UR - http://www.scopus.com/inward/record.url?scp=33748506522&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33748506522&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2958.2006.05246.x
DO - 10.1111/j.1365-2958.2006.05246.x
M3 - Article
C2 - 16771842
AN - SCOPUS:33748506522
SN - 0950-382X
VL - 61
SP - 394
EP - 406
JO - Molecular Microbiology
JF - Molecular Microbiology
IS - 2
ER -