Deterministic Absolute Negative Mobility for Micro- and Submicrometer Particles Induced in a Microfluidic Device

Jinghui Luo, Katherine A. Muratore, Edgar A. Arriaga, Alexandra Ros

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


Efficient separations of particles with micron and submicron dimensions are extremely useful in preparation and analysis of materials for nanotechnological and biological applications. Here, we demonstrate a nonintuitive, yet efficient, separation mechanism for μm and subμm colloidal particles and organelles, taking advantage of particle transport in a nonlinear post array in a microfluidic device under the periodic action of electrokinetic and dielectrophoretic forces. We reveal regimes in which deterministic particle migration opposite to the average applied force occurs for a larger particle, a typical signature of deterministic absolute negative mobility (dANM), whereas normal response is obtained for smaller particles. The coexistence of dANM and normal migration was characterized and optimized in numerical modeling and subsequently implemented in a microfluidic device demonstrating at least 2 orders of magnitude higher migration speeds as compared to previous ANM systems. We also induce dANM for mouse liver mitochondria and envision that the separation mechanisms described here provide size selectivity required in future separations of organelles, nanoparticles, and protein nanocrystals.

Original languageEnglish (US)
Pages (from-to)5920-5927
Number of pages8
JournalAnalytical Chemistry
Issue number11
StatePublished - Jun 7 2016

ASJC Scopus subject areas

  • Analytical Chemistry


Dive into the research topics of 'Deterministic Absolute Negative Mobility for Micro- and Submicrometer Particles Induced in a Microfluidic Device'. Together they form a unique fingerprint.

Cite this