Determination of torque generation from the power stroke of Escherichia coli F1-ATPase

Tassilo Hornung; Robert Ishmukhametov; David Spetzler; James Martin; Wayne Frasch

doi:10.1016/j.bbabio.2008.04.016

Determination of torque generation from the power stroke of Escherichia coli F₁-ATPase

Tassilo Hornung, Robert Ishmukhametov, David Spetzler, James Martin, Wayne Frasch

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The torque generated by the power stroke of Escherichia coli F₁-ATPase was determined as a function of the load from measurements of the velocity of the γ-subunit obtained using a 0.25 μs time resolution and direct measurements of the drag from 45 to 91 nm gold nanorods. This result was compared to values of torque calculated using four different drag models. Although the γ-subunit was able to rotate with a 20× increase in viscosity, the transition time decreased from 0.4 ms to 5.26 ms. The torque was measured to be 63 ± 8 pN nm, independent of the load on the enzyme.

Original language	English (US)
Pages (from-to)	579-582
Number of pages	4
Journal	Biochimica et Biophysica Acta - Bioenergetics
Volume	1777
Issue number	7-8
DOIs	https://doi.org/10.1016/j.bbabio.2008.04.016
State	Published - Jul 2008

Keywords

F-ATPase
Gold nanorod
High-speed data acquisition
Molecular motor
Single molecule

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

Access to Document

10.1016/j.bbabio.2008.04.016

Cite this

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title = "Determination of torque generation from the power stroke of Escherichia coli F1-ATPase",

abstract = "The torque generated by the power stroke of Escherichia coli F1-ATPase was determined as a function of the load from measurements of the velocity of the γ-subunit obtained using a 0.25 μs time resolution and direct measurements of the drag from 45 to 91 nm gold nanorods. This result was compared to values of torque calculated using four different drag models. Although the γ-subunit was able to rotate with a 20× increase in viscosity, the transition time decreased from 0.4 ms to 5.26 ms. The torque was measured to be 63 ± 8 pN nm, independent of the load on the enzyme.",

keywords = "F-ATPase, Gold nanorod, High-speed data acquisition, Molecular motor, Single molecule",

author = "Tassilo Hornung and Robert Ishmukhametov and David Spetzler and James Martin and Wayne Frasch",

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T1 - Determination of torque generation from the power stroke of Escherichia coli F1-ATPase

AU - Hornung, Tassilo

AU - Ishmukhametov, Robert

AU - Spetzler, David

AU - Martin, James

AU - Frasch, Wayne

N1 - Funding Information: This work was supported by National Institutes of Health grant GM50202 to W.D.F.

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KW - F-ATPase

KW - Gold nanorod

KW - High-speed data acquisition

KW - Molecular motor

KW - Single molecule

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