@article{b345259fe1d042faabdc561ddbee3f39,
title = "Determination of major histocompatibility class I and class II genetic composition of the Caribbean Primate Center specific pathogen-free rhesus macaque (Macaca mulatta) colony based on massively parallel sequencing",
abstract = "Background: Knowledge of major histocompatibility complex (MHC) composition and distribution in rhesus macaque colonies is critical for management strategies that maximize the utility of this model for biomedical research. Methods: Variation within the Mamu-A and Mamu-B (class I) and DRB, DQA/B, and DPA/B (class II) regions of 379 animals from the Caribbean Primate Research Center's (CPRC) specific pathogen free (SPF) colony was examined using massively parallel sequencing. Results: Analyses of the 7 MHC loci revealed a background of Indian origin with high levels of variation despite past genetic bottlenecks. All loci exhibited mutual linkage disequilibria while conforming to Hardy–Weinberg expectations suggesting the achievement of mutation-selection balance. Conclusion: The CPRC's SPF colony is a significant resource for research on AIDS and other infectious agents. Characterizing colony-wide MHC variability facilitates the breeding and selection of animals bearing desired haplotypes and increases the investigator's ability to understand the immune responses mounted by these animals.",
keywords = "Mamu haplotypes, colony genetic structure, genetic management, major histocompatibility complex genotyping, next generation sequence",
author = "Sreetharan Kanthaswamy and Oldt, {Robert F.} and Jillian Ng and Smith, {David Glenn} and Mart{\'i}nez, {Melween I.} and Sariol, {Carlos A.}",
note = "Funding Information: We thank the dedicated staff at Cayo Santiago and Sabana Seca Field Station for their continued dedication and care in support of the CPRC's animal population. We thank the WNPRC's Genetics Services Unit for performing the MPS analysis on a fee for service basis. We would like to thank WNPRC's Dr Roger Wiseman for his valuable insight into the MHC analysis and data. We are very grateful to Dr Gaby Doxiadis of the Biomedical Primate Research Centre in Rijswijk, The Netherlands, for kindly reviewing this manuscript, and providing critical comments to improve it. Animals involved in this study were managed in compliance with Institutional Animal Care and Use Committee (IACUC) regulations and with the National Institutes of Health (NIH) guidelines prescribing the humane care and use of laboratory animals. This research complies with the protocols approved by the IACUC (protocol numbers: 7890112 and 7890113) at the University of Puerto Rico and with the American Society of Primatologists (ASP) Principles for the Ethical Treatment of Nonhuman Primates. All research reported in this manuscript was performed in accordance with the legal requirements of Puerto Rico. This research was supported by NIH-ORIP grant number: 5U42OD021458-16. Funding Information: Animals involved in this study were managed in compliance with Institutional Animal Care and Use Committee (IACUC) regulations and with the National Institutes of Health (NIH) guidelines prescribing the humane care and use of laboratory animals. This research complies with the protocols approved by the IACUC (protocol numbers: 7890112 and 7890113) at the University of Puerto Rico and with the American Society of Primatologists (ASP) Principles for the Ethical Treatment of Nonhuman Primates. All research reported in this manuscript was performed in accordance with the legal requirements of Puerto Rico. This research was supported by NIH-ORIP grant number: 5U42OD021458-16. Publisher Copyright: {\textcopyright} 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd",
year = "2018",
month = dec,
doi = "10.1111/jmp.12353",
language = "English (US)",
volume = "47",
pages = "379--387",
journal = "Journal of Medical Primatology",
issn = "0047-2565",
publisher = "Blackwell Munksgaard",
number = "6",
}