Detecting Necroptosis in Virus-Infected Cells

Samantha M. Cotsmire, Mateusz Szczerba, Bertram L. Jacobs

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations


Necroptosis has been implicated as a critical cell death pathway in cancers, Alzheimer’s and other neurodegenerative diseases, and virus-infected cells. Necroptosis occurs when mixed-lineage kinase domain-like protein (MLKL) punctures the cytoplasmic membrane allowing a rapid influx of water leading to a loss of cellular integrity. As its role in human disease becomes apparent, methods identifying necroptosis will need to be further developed and optimized. Here we describe identification of necroptosis through quantifying cell death with pathway inhibitors and using western blots to identify end points of MLKL activation and protein-protein interactions leading to it.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages18
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Cell death
  • DSP-cross-linking
  • Dye exclusion
  • Immunoblots
  • Necroptosis
  • Vaccinia

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


Dive into the research topics of 'Detecting Necroptosis in Virus-Infected Cells'. Together they form a unique fingerprint.

Cite this