Design and expression of a short peptide as an HIV detection probe

Jamie A. Lines; Zhiqiang Yu; Larisa Dedkova; Shengxi Chen

doi:10.1016/j.bbrc.2013.11.095

Design and expression of a short peptide as an HIV detection probe

Jamie A. Lines, Zhiqiang Yu, Larisa Dedkova, Shengxi Chen

Bioenergetics, Center for (BE)

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

To explore a low-cost novel probe for HIV detection, we designed and prepared a 50-amino acid-length short fusion peptide (FP-50) via Escherichia coli in vivo expression. It was employed as a novel probe to detect HIV-1 gp120 protein. The detectable level of gp120 protein using the FP-50 peptide was approximately 20-200 times lower than previously published methods that used a pair of monoclonal antibodies. Thus, this short peptide is a very promising component for detection of gp120 protein during early stages of HIV infection.

Original language	English (US)
Pages (from-to)	308-312
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	443
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2013.11.095
State	Published - Jan 3 2014

Keywords

Early stages
Fusion peptide
Gp120
HIV detection
In vivo expression

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2013.11.095

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title = "Design and expression of a short peptide as an HIV detection probe",

abstract = "To explore a low-cost novel probe for HIV detection, we designed and prepared a 50-amino acid-length short fusion peptide (FP-50) via Escherichia coli in vivo expression. It was employed as a novel probe to detect HIV-1 gp120 protein. The detectable level of gp120 protein using the FP-50 peptide was approximately 20-200 times lower than previously published methods that used a pair of monoclonal antibodies. Thus, this short peptide is a very promising component for detection of gp120 protein during early stages of HIV infection.",

keywords = "Early stages, Fusion peptide, Gp120, HIV detection, In vivo expression",

author = "Lines, {Jamie A.} and Zhiqiang Yu and Larisa Dedkova and Shengxi Chen",

note = "Funding Information: We thank Dr. Sidney M. Hecht for his support. This work was performed with the support of Bill & Melinda Gates Foundation through the Grant Challenges Explorations initiative (Grant No. OPP1061337 ). ",

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AU - Lines, Jamie A.

AU - Yu, Zhiqiang

AU - Dedkova, Larisa

AU - Chen, Shengxi

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AB - To explore a low-cost novel probe for HIV detection, we designed and prepared a 50-amino acid-length short fusion peptide (FP-50) via Escherichia coli in vivo expression. It was employed as a novel probe to detect HIV-1 gp120 protein. The detectable level of gp120 protein using the FP-50 peptide was approximately 20-200 times lower than previously published methods that used a pair of monoclonal antibodies. Thus, this short peptide is a very promising component for detection of gp120 protein during early stages of HIV infection.

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KW - In vivo expression

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Design and expression of a short peptide as an HIV detection probe

Abstract

Keywords

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