TY - JOUR
T1 - Defocus as an ineffective means of changing spot size for fluctuation microscopy
AU - Gibson, J. M.
AU - Treacy, Michael
PY - 2009
Y1 - 2009
N2 - We examine the use of defocus for changing the effective spot size in fluctuation microscopy. Our conclusions relate to other coherent techniques. While it is possible to increase the illuminated spot size by defocusing, the number of phase oscillations inside the illuminated spot increases. As a result the stationary phase region increases with defocus at a slower rate than the spot size. Therefore, the effective spot size for fluctuation microscopy is not the beam size, and the phase interference is increasingly diluted by incoherent contributions, rendering defocus an ineffective means of changing the spot size for fluctuation microscopy.
AB - We examine the use of defocus for changing the effective spot size in fluctuation microscopy. Our conclusions relate to other coherent techniques. While it is possible to increase the illuminated spot size by defocusing, the number of phase oscillations inside the illuminated spot increases. As a result the stationary phase region increases with defocus at a slower rate than the spot size. Therefore, the effective spot size for fluctuation microscopy is not the beam size, and the phase interference is increasingly diluted by incoherent contributions, rendering defocus an ineffective means of changing the spot size for fluctuation microscopy.
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U2 - 10.1088/1742-6596/186/1/012053
DO - 10.1088/1742-6596/186/1/012053
M3 - Article
AN - SCOPUS:73649134702
SN - 1742-6588
VL - 186
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
M1 - 012053
ER -