The interior of a living cell is very distinct from dilute aqueous solutions that are often used to study the function of proteins and enzymes experimentally. Here, we discuss novel experimental techniques and modeling approaches that provide a deeper understanding of the effects of crowding, intermolecular interactions, and the influence of osmolytes in realistic biological environments, including cells. Further, we discuss adaptation mechanisms involving in-cell solvation environments to extreme conditions, such as high pressure in the deep sea, and their relevance to biotechnology.
|Translated title of the contribution||The interior of the cell: A complex solvent. Biochemical reactions under physiological and extreme conditions|
|Number of pages||8|
|Journal||Chemie in Unserer Zeit|
|State||Published - Feb 1 2017|
ASJC Scopus subject areas