Abstract
Background: Biocatalysts provide an economical and energy-efficient alternative to traditional chemical manufacturing processes. For processes where biocatalysts currently do not exist or existing protein catalysts function poorly, there is a tremendous need to discover new protein catalysts that function in industrial settings. The protein engineering community has traditionally relied on cell-based techniques in 96-well format to evolve new catalysts or improve existing enzymes. Objective: This review examines recent progress made in many display technologies, providing powerful alternatives for generating novel enzymes with altered specificity or altogether new types of function. Methods: Library creation methods and display technologies that are commonly used in conjunction with enzyme evolution are discussed. Conclusion: We conclude with an expert opinion on future trans-disciplinary approaches that combine directed evolution with computational design as novel platforms for rapidly discovering new types of catalytic function.
Original language | English (US) |
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Pages (from-to) | 1087-1098 |
Number of pages | 12 |
Journal | Expert Opinion on Biological Therapy |
Volume | 8 |
Issue number | 8 |
DOIs | |
State | Published - Aug 2008 |
Keywords
- Directed evolution
- Library diversity
- Protein biocatalyst
ASJC Scopus subject areas
- Pharmacology
- Drug Discovery
- Clinical Biochemistry