@article{15fc4435393a4ee29dea67dbc012d07c,
title = "Coumarin luciferins and mutant luciferases for robust multi-component bioluminescence imaging",
abstract = "Multi-component bioluminescence imaging requires an expanded collection of luciferase-luciferin pairs that emit far-red or near-infrared light. Toward this end, we prepared a new class of luciferins based on a red-shifted coumarin scaffold. These probes (CouLuc-1s) were accessed in a two-step sequenceviadirect modification of commercial dyes. The bioluminescent properties of the CouLuc-1 analogs were also characterized, and complementary luciferase enzymes were identified using a two-pronged screening strategy. The optimized enzyme-substrate pairs displayed robust photon outputs and emitted a significant portion of near-infrared light. The CouLuc-1 scaffolds are also structurally distinct from existing probes, enabling rapid multi-component imaging. Collectively, this work provides novel bioluminescent tools along with a blueprint for crafting additional fluorophore-derived probes for multiplexed imaging.",
author = "Zi Yao and Caldwell, {Donald R.} and Love, {Anna C.} and Bethany Kolbaba-Kartchner and Mills, {Jeremy H.} and Schnermann, {Martin J.} and Prescher, {Jennifer A.}",
note = "Funding Information: This work was supported by the U.S. National Institutes of Health (R01 GM107630 to J. A. P.) and the Intramural Research Program of the National Institutes of Health (NIH), NCI-CCR. Z. Y. was supported by the National Science Foundation via the BEST IGERT (DGE-1144901) program and a Graduate Research Fellowship (DGE-1321846). A. L. was supported by the National Science Foundation via the BEST IGERT (DGE-1144901). We thank Dr. Funding Information: This work was supported by the U.S. National Institutes of Health (R01 GM107630 to J. A. P.) and the Intramural Research Program of the National Institutes of Health (NIH), NCI-CCR. Z. Y. was supported by the National Science Foundationviathe BEST IGERT (DGE-1144901) program and a Graduate Research Fellowship (DGE-1321846). A. L. was supported by the National Science Foundationviathe BEST IGERT (DGE-1144901). We thank Dr. Joseph Barchi, NCI Center for Cancer Research (NCI-CCR) for NMR assistance and Dr. James Kelley, NCI-CCR, for mass spectrometry analysis. Some experiments were performed at the Laser Spectroscopy labs (LSL) at UCI. We thank Caroline Brennan for assisting with image processing, Anastasia Ionkina for cell line generation, along with other members of the Prescher lab for helpful discussions. We also thank members of the Weiss and Martin laboratories (UCI) for providing equipment and reagents. Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2021.",
year = "2021",
month = sep,
day = "21",
doi = "10.1039/d1sc03114g",
language = "English (US)",
volume = "12",
pages = "11684--11691",
journal = "Chemical Science",
issn = "2041-6520",
publisher = "Royal Society of Chemistry",
number = "35",
}