TY - JOUR
T1 - Copper nanoparticles toxicity
T2 - Laboratory strains verses environmental bacterial isolates
AU - Alum, Absar
AU - Alboloushi, Ali
AU - Abbaszadegan, Morteza
N1 - Funding Information:
Funding for this work was provided by the National Science Foundation Water and Environmental Technology Center at Arizona State University. NSF grant # 0855802 and ASU # XUS0001.
Funding Information:
This work was supported by the National Science Foundation Water and Environmental Technology Center at Arizona State University, (NSF 0855802, ASU XUS0001).
PY - 2018/6/7
Y1 - 2018/6/7
N2 - Nanoparticles have emerged as significant environmental contaminants and their impact has been studied using laboratory strains of bacteria. This study focuses on investigating the response of environmental isolate and laboratory strains of E. coli to 50 and 100 nm size of copper nanoparticles (CuNPs). The laboratory cultures included pathogenic and non-pathogenic strains. The environmental isolate and the non-pathogenic E. coli strain showed different inactivation patterns. After 2 h exposure to 50 nm CuNPs, the environmental isolate and the lab strain of E. coli lost 7.22 and 6.47 log; whereas the reduction of 6.16 and 6.68 log resulted after exposure to 100 nm CuNPs, respectively. The pathogenic E. coli O157:H7 exposed to 50 and 100 nm CuNPs for 2 h resulted in 5.24 and 6.54 log reduction, respectively. Although the environmental isolate and the laboratory strains of E. coli showed similar inactivation trends; they exhibited different toxicity elicitation mechanisms after exposure to the CuNPs. The pathogenic and non-pathogenic strains elicited significantly different levels of glutathione reductase (GR) activities, an enzyme critical for protection against radicals. Similarly, the environmental isolate and the lab strains of E. coli exhibited opposite trend in GR activities. These results clearly indicate divergence in the toxicity elicitation in the environmental isolate versus the laboratory strains from exposure to CuNPs, which highlights the need for an in-depth investigation of the impact of NPs on the biological processes and long-term effect of high load of NPs on the stability of aquatic and terrestrial ecologies.
AB - Nanoparticles have emerged as significant environmental contaminants and their impact has been studied using laboratory strains of bacteria. This study focuses on investigating the response of environmental isolate and laboratory strains of E. coli to 50 and 100 nm size of copper nanoparticles (CuNPs). The laboratory cultures included pathogenic and non-pathogenic strains. The environmental isolate and the non-pathogenic E. coli strain showed different inactivation patterns. After 2 h exposure to 50 nm CuNPs, the environmental isolate and the lab strain of E. coli lost 7.22 and 6.47 log; whereas the reduction of 6.16 and 6.68 log resulted after exposure to 100 nm CuNPs, respectively. The pathogenic E. coli O157:H7 exposed to 50 and 100 nm CuNPs for 2 h resulted in 5.24 and 6.54 log reduction, respectively. Although the environmental isolate and the laboratory strains of E. coli showed similar inactivation trends; they exhibited different toxicity elicitation mechanisms after exposure to the CuNPs. The pathogenic and non-pathogenic strains elicited significantly different levels of glutathione reductase (GR) activities, an enzyme critical for protection against radicals. Similarly, the environmental isolate and the lab strains of E. coli exhibited opposite trend in GR activities. These results clearly indicate divergence in the toxicity elicitation in the environmental isolate versus the laboratory strains from exposure to CuNPs, which highlights the need for an in-depth investigation of the impact of NPs on the biological processes and long-term effect of high load of NPs on the stability of aquatic and terrestrial ecologies.
KW - E. coli
KW - Nanoparticles
KW - environmental isolate
KW - lab strain
KW - toxicity mechanism
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U2 - 10.1080/10934529.2018.1429727
DO - 10.1080/10934529.2018.1429727
M3 - Article
C2 - 29381417
AN - SCOPUS:85041202811
SN - 1093-4529
VL - 53
SP - 643
EP - 650
JO - Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
JF - Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
IS - 7
ER -