Pyridine and its heterocyclic derivatives are widely encountered in industrial wastewaters, and they are relatively recalcitrant to biodegradation. Pyridine biodegradation is initiated by two mono-oxygenation reactions that compete for intracellular electron donor (2H). In our experiments, UV photolysis of pyridine generated succinate, whose oxidation augmented the intracellular electron donor and accelerated pyridine biodegradation and mineralization. The first mono-oxygenation reaction always was faster than the second one, because electrons provided by intracellular electron donors were preferentially utilized by the first mono-oxygenase; this was true even when the concentration of 2HP was greater than the concentration of pyridine. In addition, the first mono-oxygenation had faster kinetics because it had higher affinity for its substrate (pyridine), along with less substrate self-inhibition.

Original languageEnglish (US)
Pages (from-to)419-427
Number of pages9
Issue number5
StatePublished - Oct 1 2018


  • 2-Hydroxypyridine
  • Competition for electrons
  • Mono-oxygenation
  • Pyridine

ASJC Scopus subject areas

  • Pollution
  • Bioengineering
  • Environmental Engineering
  • Microbiology
  • Environmental Chemistry


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