Comparative Genomics of Closely-Related Gordonia Cluster DR Bacteriophages

Cyril J. Versoza; Abigail A. Howell; Tanya Aftab; Madison Blanco; Akarshi Brar; Elaine Chaffee; Nicholas Howell; Willow Leach; Jackelyn Lobatos; Michael Luca; Meghna Maddineni; Ruchira Mirji; Corinne Mitra; Maria Strasser; Saige Munig; Zeel Patel; Minerva So; Makena Sy; Sarah Weiss; Susanne P. Pfeifer

doi:10.3390/v14081647

Comparative Genomics of Closely-Related Gordonia Cluster DR Bacteriophages

Cyril J. Versoza, Abigail A. Howell, Tanya Aftab, Madison Blanco, Akarshi Brar, Elaine Chaffee, Nicholas Howell, Willow Leach, Jackelyn Lobatos, Michael Luca, Meghna Maddineni, Ruchira Mirji, Corinne Mitra, Maria Strasser, Saige Munig, Zeel Patel, Minerva So, Makena Sy, Sarah Weiss, Susanne P. Pfeifer

Life Sciences, School of (SOLS)

Research output: Contribution to journal › Article › peer-review

Abstract

Bacteriophages infecting bacteria of the genus Gordonia have increasingly gained interest in the scientific community for their diverse applications in agriculture, biotechnology, and medicine, ranging from biocontrol agents in wastewater management to the treatment of opportunistic pathogens in pulmonary disease patients. However, due to the time and costs associated with experimental isolation and cultivation, host ranges for many bacteriophages remain poorly characterized, hindering a more efficient usage of bacteriophages in these areas. Here, we perform a series of computational genomic inferences to predict the putative host ranges of all Gordonia cluster DR bacteriophages known to date. Our analyses suggest that BiggityBass (as well as several of its close relatives) is likely able to infect host bacteria from a wide range of genera—from Gordonia to Nocardia to Rhodococcus, making it a suitable candidate for future phage therapy and wastewater treatment strategies.

Original language	English (US)
Article number	1647
Journal	Viruses
Volume	14
Issue number	8
DOIs	https://doi.org/10.3390/v14081647
State	Published - Aug 2022

Keywords

Gordonia
bacteriophage
cluster DR
comparative genomics
host range

ASJC Scopus subject areas

Infectious Diseases
Virology

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10.3390/v14081647

Cite this

Versoza, C. J., Howell, A. A., Aftab, T., Blanco, M., Brar, A., Chaffee, E., Howell, N., Leach, W., Lobatos, J., Luca, M., Maddineni, M., Mirji, R., Mitra, C., Strasser, M., Munig, S., Patel, Z., So, M., Sy, M., Weiss, S., & Pfeifer, S. P. (2022). Comparative Genomics of Closely-Related Gordonia Cluster DR Bacteriophages. Viruses, 14(8), Article 1647. https://doi.org/10.3390/v14081647

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title = "Comparative Genomics of Closely-Related Gordonia Cluster DR Bacteriophages",

abstract = "Bacteriophages infecting bacteria of the genus Gordonia have increasingly gained interest in the scientific community for their diverse applications in agriculture, biotechnology, and medicine, ranging from biocontrol agents in wastewater management to the treatment of opportunistic pathogens in pulmonary disease patients. However, due to the time and costs associated with experimental isolation and cultivation, host ranges for many bacteriophages remain poorly characterized, hindering a more efficient usage of bacteriophages in these areas. Here, we perform a series of computational genomic inferences to predict the putative host ranges of all Gordonia cluster DR bacteriophages known to date. Our analyses suggest that BiggityBass (as well as several of its close relatives) is likely able to infect host bacteria from a wide range of genera—from Gordonia to Nocardia to Rhodococcus, making it a suitable candidate for future phage therapy and wastewater treatment strategies.",

keywords = "Gordonia, bacteriophage, cluster DR, comparative genomics, host range",

author = "Versoza, {Cyril J.} and Howell, {Abigail A.} and Tanya Aftab and Madison Blanco and Akarshi Brar and Elaine Chaffee and Nicholas Howell and Willow Leach and Jackelyn Lobatos and Michael Luca and Meghna Maddineni and Ruchira Mirji and Corinne Mitra and Maria Strasser and Saige Munig and Zeel Patel and Minerva So and Makena Sy and Sarah Weiss and Pfeifer, {Susanne P.}",

note = "Funding Information: This research was funded by a National Science Foundation CAREER grant to SPP, grant number DEB-2045343. Publisher Copyright: {\textcopyright} 2022 by the authors.",

year = "2022",

month = aug,

doi = "10.3390/v14081647",

language = "English (US)",

volume = "14",

journal = "Viruses",

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AU - Howell, Abigail A.

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AU - Blanco, Madison

AU - Brar, Akarshi

AU - Chaffee, Elaine

AU - Howell, Nicholas

AU - Leach, Willow

AU - Lobatos, Jackelyn

AU - Luca, Michael

AU - Maddineni, Meghna

AU - Mirji, Ruchira

AU - Mitra, Corinne

AU - Strasser, Maria

AU - Munig, Saige

AU - Patel, Zeel

AU - So, Minerva

AU - Sy, Makena

AU - Weiss, Sarah

AU - Pfeifer, Susanne P.

PY - 2022/8

Y1 - 2022/8

N2 - Bacteriophages infecting bacteria of the genus Gordonia have increasingly gained interest in the scientific community for their diverse applications in agriculture, biotechnology, and medicine, ranging from biocontrol agents in wastewater management to the treatment of opportunistic pathogens in pulmonary disease patients. However, due to the time and costs associated with experimental isolation and cultivation, host ranges for many bacteriophages remain poorly characterized, hindering a more efficient usage of bacteriophages in these areas. Here, we perform a series of computational genomic inferences to predict the putative host ranges of all Gordonia cluster DR bacteriophages known to date. Our analyses suggest that BiggityBass (as well as several of its close relatives) is likely able to infect host bacteria from a wide range of genera—from Gordonia to Nocardia to Rhodococcus, making it a suitable candidate for future phage therapy and wastewater treatment strategies.

AB - Bacteriophages infecting bacteria of the genus Gordonia have increasingly gained interest in the scientific community for their diverse applications in agriculture, biotechnology, and medicine, ranging from biocontrol agents in wastewater management to the treatment of opportunistic pathogens in pulmonary disease patients. However, due to the time and costs associated with experimental isolation and cultivation, host ranges for many bacteriophages remain poorly characterized, hindering a more efficient usage of bacteriophages in these areas. Here, we perform a series of computational genomic inferences to predict the putative host ranges of all Gordonia cluster DR bacteriophages known to date. Our analyses suggest that BiggityBass (as well as several of its close relatives) is likely able to infect host bacteria from a wide range of genera—from Gordonia to Nocardia to Rhodococcus, making it a suitable candidate for future phage therapy and wastewater treatment strategies.

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KW - bacteriophage

KW - cluster DR

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KW - host range

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Comparative Genomics of Closely-Related Gordonia Cluster DR Bacteriophages

Abstract

Keywords

ASJC Scopus subject areas

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