Chemical modifications of transfer RNA species. Heavy atom derivatization of aminoacyl tRNA

Francis J. Schmidt; Robert M. Bock; Sidney M. Hecht

doi:10.1016/S0006-291X(72)80072-X

Chemical modifications of transfer RNA species. Heavy atom derivatization of aminoacyl tRNA

Francis J. Schmidt, Robert M. Bock, Sidney M. Hecht

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Specific heavy atom derivatization of valyl and arginyl tRNA's from E. coli has been effected by the use of the N-hydroxysuccinimide esters of certain carboxylic acids. The derivatized tRNA's have been separated from underivatized material and shown to be stable under the conditions required for crystallization.

Original language	English (US)
Pages (from-to)	451-456
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	48
Issue number	2
DOIs	https://doi.org/10.1016/S0006-291X(72)80072-X
State	Published - Jul 25 1972
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/S0006-291X(72)80072-X

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title = "Chemical modifications of transfer RNA species. Heavy atom derivatization of aminoacyl tRNA",

abstract = "Specific heavy atom derivatization of valyl and arginyl tRNA's from E. coli has been effected by the use of the N-hydroxysuccinimide esters of certain carboxylic acids. The derivatized tRNA's have been separated from underivatized material and shown to be stable under the conditions required for crystallization.",

author = "Schmidt, {Francis J.} and Bock, {Robert M.} and Hecht, {Sidney M.}",

note = "Funding Information: We thank Drs. A.H. Mehler and K, Chakraburrty for help with the initial experiments. We thank Dr. M. Miyazaki for the preparation of BD-cellulose and M. A. Helmes for technical assistance. Supported by NIH research grant GM-12395 and NSF research grant GB-31212. FJS is an NIH trainee.",

year = "1972",

month = jul,

day = "25",

doi = "10.1016/S0006-291X(72)80072-X",

language = "English (US)",

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journal = "Biochemical and Biophysical Research Communications",

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T1 - Chemical modifications of transfer RNA species. Heavy atom derivatization of aminoacyl tRNA

AU - Schmidt, Francis J.

AU - Bock, Robert M.

AU - Hecht, Sidney M.

N1 - Funding Information: We thank Drs. A.H. Mehler and K, Chakraburrty for help with the initial experiments. We thank Dr. M. Miyazaki for the preparation of BD-cellulose and M. A. Helmes for technical assistance. Supported by NIH research grant GM-12395 and NSF research grant GB-31212. FJS is an NIH trainee.

PY - 1972/7/25

Y1 - 1972/7/25

N2 - Specific heavy atom derivatization of valyl and arginyl tRNA's from E. coli has been effected by the use of the N-hydroxysuccinimide esters of certain carboxylic acids. The derivatized tRNA's have been separated from underivatized material and shown to be stable under the conditions required for crystallization.

AB - Specific heavy atom derivatization of valyl and arginyl tRNA's from E. coli has been effected by the use of the N-hydroxysuccinimide esters of certain carboxylic acids. The derivatized tRNA's have been separated from underivatized material and shown to be stable under the conditions required for crystallization.

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U2 - 10.1016/S0006-291X(72)80072-X

DO - 10.1016/S0006-291X(72)80072-X

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AN - SCOPUS:0015523350

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Chemical modifications of transfer RNA species. Heavy atom derivatization of aminoacyl tRNA

Abstract

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