Charge Sensitive Optical Detection for Measurement of Small-Molecule Binding Kinetics

Shaopeng Wang, Guangzhong Ma, Runli Liang, Nongjian Tao

Research output: Chapter in Book/Report/Conference proceedingChapter


Charge sensitive optical detection (CSOD) technique is a label-free method for real-time measurement of molecular interactions. Traditional label-free optical detection techniques mostly measure the mass of a molecule, and they are less sensitive to small molecules. In contrast, CSOD detects the charge of a molecule, where the signal does not diminish with the size of the molecule, thus capable for studying small molecules. In addition, CSOD is compatible with the standard microplate platform, making it suitable for high-throughput screening of drug candidates. In CSOD, an optical fiber functionalized with the probe molecule is dipped into a well of a microplate where an alternate perpendicular electrical field is applied to the fiber, which drives the fiber into oscillation because of the presence of surface charge on the fiber. The binding of the target molecules changes the charge of the fiber, and thus the amplitude and phase of the oscillating fiber, which are precisely measured through tracking of the optical images of the fiber tip.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages14
StatePublished - 2022

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Binding kinetics
  • CSOD
  • Charge sensitive optical detection
  • Label free detection
  • Optical fiber
  • Optical imaging
  • Small molecule

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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