TY - JOUR
T1 - Characterization of Iranian Tomato aspermy virus isolates with a variant 2b gene sequence
AU - Maddahian, Mohammad
AU - Massumi, Hossein
AU - Heydarnejad, Jahangir
AU - Hosseini Pour, Akbar
AU - Varsani, Arvind
N1 - Funding Information:
The sequences reported in this manuscript have been deposited in GenBank, with access numbers KT757536–38, JQ403612–14, KT757534–35 and KT757539–40. The authors declare that they have no conflict of interest. This work was supported by a grant from Shahid Bahonar University of Kerman, Kerman, Iran. (Grant number 513614).
Publisher Copyright:
© 2017, Sociedade Brasileira de Fitopatologia.
PY - 2017/12/1
Y1 - 2017/12/1
N2 - Three isolates of Tomato aspermy virus (TAV) were identified from two ornamental plants, Petunia hybrida and Chrysanthemum morifolium, sampled in Kerman province in the southeastern region of Iran. The complete genome of one isolate (Ker.Mah.P) and three open reading frames (ORFs), encoding coat protein (CP), movement protein (MP) and 2b protein of the three isolates were sequenced. The three genome segments of the Ker.Mah.P isolate (RNA-1, −2 and −3) are comprised of 3351, 3035 and 2194 nts, respectively. The nucleotide sequences of the three Iranian isolates were compared with other available sequences in GenBank, and the genome of Ker.Mah.P showed >96% sequence identity with sequences of TAV isolates. In a host range study, the three Iranian isolates induced chlorotic and necrotic symptoms on the true leaves of Cucumis sativus cv. Maximum F4 and systemic mottle and necrotic local lesions on Chenopodium amaranticolor, whereas other isolates whose sequences are deposited in GenBank cause only necrotic local lesions on these test plants. Sequences of the 2b gene of the three Iranian and one Chinese isolate have an additional 27 nt insert and it is possible that this domain, which adds nine amino acids to the 2b protein, may affect the biological properties of the virus. To the best of our knowledge, this is the first report of a TAV isolate from the Middle East whose genome has been sequenced, as well as the first global report of the natural occurrence of TAV in P. hybrida.
AB - Three isolates of Tomato aspermy virus (TAV) were identified from two ornamental plants, Petunia hybrida and Chrysanthemum morifolium, sampled in Kerman province in the southeastern region of Iran. The complete genome of one isolate (Ker.Mah.P) and three open reading frames (ORFs), encoding coat protein (CP), movement protein (MP) and 2b protein of the three isolates were sequenced. The three genome segments of the Ker.Mah.P isolate (RNA-1, −2 and −3) are comprised of 3351, 3035 and 2194 nts, respectively. The nucleotide sequences of the three Iranian isolates were compared with other available sequences in GenBank, and the genome of Ker.Mah.P showed >96% sequence identity with sequences of TAV isolates. In a host range study, the three Iranian isolates induced chlorotic and necrotic symptoms on the true leaves of Cucumis sativus cv. Maximum F4 and systemic mottle and necrotic local lesions on Chenopodium amaranticolor, whereas other isolates whose sequences are deposited in GenBank cause only necrotic local lesions on these test plants. Sequences of the 2b gene of the three Iranian and one Chinese isolate have an additional 27 nt insert and it is possible that this domain, which adds nine amino acids to the 2b protein, may affect the biological properties of the virus. To the best of our knowledge, this is the first report of a TAV isolate from the Middle East whose genome has been sequenced, as well as the first global report of the natural occurrence of TAV in P. hybrida.
KW - 2b gene
KW - Iran
KW - Tomato aspermy virus
KW - complete genome
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U2 - 10.1007/s40858-017-0173-1
DO - 10.1007/s40858-017-0173-1
M3 - Article
AN - SCOPUS:85035242764
SN - 1982-5676
VL - 42
SP - 475
EP - 484
JO - Tropical Plant Pathology
JF - Tropical Plant Pathology
IS - 6
ER -