Cellular-level near-wall unsteadiness of high-hematocrit erythrocyte flow using confocal μpIV

Michael J. Patrick; Chia Yuan Chen; David Frakes; Onur Dur; Kerem Pekkan

doi:10.1007/s00348-010-0943-8

Cellular-level near-wall unsteadiness of high-hematocrit erythrocyte flow using confocal μpIV

Michael J. Patrick, Chia Yuan Chen, David Frakes, Onur Dur, Kerem Pekkan

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

In hemodynamics, the inherent intermittency of two-phase cellular-level flow has received little attention. Unsteadiness is reported and quantified for the first time in the literature using a combination of fluorescent dye labeling, time-resolved scanning confocal microscopy, and micro-particle image velocimetry (μPIV). The near-wall red blood cell (RBC) motion of physiologic high-hematocrit blood in a rectangular microchannel was investigated under pressure-driven flow. Intermittent flow was associated with (1) the stretching of RBCs as they passed through RBC clusters with twisting motions; (2) external flow through local obstacles; and (3) transitionary rouleaux formations. Velocity profiles are presented for these cases. Unsteady flow clustered in local regions. Extra-cellular fluid flow generated by individual RBCs was examined using submicron fluorescent microspheres. The capabilities of confocal μPIV post-processing were verified using synthetic raw PIV data for validation. Cellular interactions and oscillating velocity profiles are presented, and 3D data are made available for computational model validation.

Original language	English (US)
Pages (from-to)	887-904
Number of pages	18
Journal	Experiments in Fluids
Volume	50
Issue number	4
DOIs	https://doi.org/10.1007/s00348-010-0943-8
State	Published - Apr 2011

ASJC Scopus subject areas

Computational Mechanics
Mechanics of Materials
Physics and Astronomy(all)
Fluid Flow and Transfer Processes

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10.1007/s00348-010-0943-8

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@article{e03c4b0b844d4c63ae2c957ede4b5691,

title = "Cellular-level near-wall unsteadiness of high-hematocrit erythrocyte flow using confocal μpIV",

abstract = "In hemodynamics, the inherent intermittency of two-phase cellular-level flow has received little attention. Unsteadiness is reported and quantified for the first time in the literature using a combination of fluorescent dye labeling, time-resolved scanning confocal microscopy, and micro-particle image velocimetry (μPIV). The near-wall red blood cell (RBC) motion of physiologic high-hematocrit blood in a rectangular microchannel was investigated under pressure-driven flow. Intermittent flow was associated with (1) the stretching of RBCs as they passed through RBC clusters with twisting motions; (2) external flow through local obstacles; and (3) transitionary rouleaux formations. Velocity profiles are presented for these cases. Unsteady flow clustered in local regions. Extra-cellular fluid flow generated by individual RBCs was examined using submicron fluorescent microspheres. The capabilities of confocal μPIV post-processing were verified using synthetic raw PIV data for validation. Cellular interactions and oscillating velocity profiles are presented, and 3D data are made available for computational model validation.",

author = "Patrick, {Michael J.} and Chen, {Chia Yuan} and David Frakes and Onur Dur and Kerem Pekkan",

note = "Funding Information: The study was partially supported through NSF CAREER 0954465. This work was done using a microscope facility funded by NSF DMR-0619424. Dr. Mohammed Islam and Dr. Deniz Kaya are acknowledged for their assistance with the microscope; Dr. Nadine Aubrey and Dr. Kerem Uguz of Mechanical Engineering (CMU) for microchannel design and fabrication. Dr. Alan Rosenbloom, Susan Andreko, Dr. James Fitzpatrick, Dr. Lauren Ernst, Dr. Byron Ballou, and Dr. Alan Waggoner are acknowledged for providing hardware, fluorescent labeling, imaging expertise, and practical advice; Dr. Bradley Keller for phlebotomy and medical expertise; Richard Prevost and Dr. Callum Gray of LaVision, Inc. for PIV processing guidance. Dr. Eser Pekkan is acknowledged for inspiring Dr. Kerem Pekkan{\textquoteright}s interest in blood cells during early childhood and for his help during the preliminary auto-fluorescence confocal microscopy experiments.",

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AU - Chen, Chia Yuan

AU - Frakes, David

AU - Dur, Onur

AU - Pekkan, Kerem

N1 - Funding Information: The study was partially supported through NSF CAREER 0954465. This work was done using a microscope facility funded by NSF DMR-0619424. Dr. Mohammed Islam and Dr. Deniz Kaya are acknowledged for their assistance with the microscope; Dr. Nadine Aubrey and Dr. Kerem Uguz of Mechanical Engineering (CMU) for microchannel design and fabrication. Dr. Alan Rosenbloom, Susan Andreko, Dr. James Fitzpatrick, Dr. Lauren Ernst, Dr. Byron Ballou, and Dr. Alan Waggoner are acknowledged for providing hardware, fluorescent labeling, imaging expertise, and practical advice; Dr. Bradley Keller for phlebotomy and medical expertise; Richard Prevost and Dr. Callum Gray of LaVision, Inc. for PIV processing guidance. Dr. Eser Pekkan is acknowledged for inspiring Dr. Kerem Pekkan’s interest in blood cells during early childhood and for his help during the preliminary auto-fluorescence confocal microscopy experiments.

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N2 - In hemodynamics, the inherent intermittency of two-phase cellular-level flow has received little attention. Unsteadiness is reported and quantified for the first time in the literature using a combination of fluorescent dye labeling, time-resolved scanning confocal microscopy, and micro-particle image velocimetry (μPIV). The near-wall red blood cell (RBC) motion of physiologic high-hematocrit blood in a rectangular microchannel was investigated under pressure-driven flow. Intermittent flow was associated with (1) the stretching of RBCs as they passed through RBC clusters with twisting motions; (2) external flow through local obstacles; and (3) transitionary rouleaux formations. Velocity profiles are presented for these cases. Unsteady flow clustered in local regions. Extra-cellular fluid flow generated by individual RBCs was examined using submicron fluorescent microspheres. The capabilities of confocal μPIV post-processing were verified using synthetic raw PIV data for validation. Cellular interactions and oscillating velocity profiles are presented, and 3D data are made available for computational model validation.

AB - In hemodynamics, the inherent intermittency of two-phase cellular-level flow has received little attention. Unsteadiness is reported and quantified for the first time in the literature using a combination of fluorescent dye labeling, time-resolved scanning confocal microscopy, and micro-particle image velocimetry (μPIV). The near-wall red blood cell (RBC) motion of physiologic high-hematocrit blood in a rectangular microchannel was investigated under pressure-driven flow. Intermittent flow was associated with (1) the stretching of RBCs as they passed through RBC clusters with twisting motions; (2) external flow through local obstacles; and (3) transitionary rouleaux formations. Velocity profiles are presented for these cases. Unsteady flow clustered in local regions. Extra-cellular fluid flow generated by individual RBCs was examined using submicron fluorescent microspheres. The capabilities of confocal μPIV post-processing were verified using synthetic raw PIV data for validation. Cellular interactions and oscillating velocity profiles are presented, and 3D data are made available for computational model validation.

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Cellular-level near-wall unsteadiness of high-hematocrit erythrocyte flow using confocal μpIV

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