TY - JOUR
T1 - Cell disruption by cationic surfactants affects bioproduct recovery from Synechocystis sp. PCC 6803
AU - Lai, Yen Jung Sean
AU - Zhou, Yun
AU - Eustance, Everett
AU - Straka, Levi
AU - Wang, Zhaocheng
AU - Rittmann, Bruce
N1 - Funding Information:
This work was supported by LightWorks , Arizona State University , the National Key Research and Development Program of China ( 2017YFC0403403 ), and the Shanghai Tongji Gao Tingyao Environmental Science & Technology Development Foundation . We thank Dr. Willem Vermaas and his laboratory in the School of Life Sciences at Arizona State University for providing Synechocystis sp. PCC6803 wild type; Dr. Dong Fu at the Center of Infectious Diseases and Vaccinology, Biodesign Institute at Arizona State University, for her expertise in flow cytometry for sample quantification; and Mr. David Lowry at the Electron Microscopy facility at the School of Life Sciences (SoLS) at Arizona State University for his expertise in sample preparation and use of the TEM.
PY - 2018/9
Y1 - 2018/9
N2 - Cationic surfactants can be used to improve extraction of high-value products from microalgae, but the best way to apply cationic surfactants has not been established. We evaluated the impacts of contact time and concentration for two cationic surfactants – dodecyltrimethylammonium bromide (DTAB) and hexadecyltrimethylammonium bromide (CTAB) – on extraction of lipids and nonpolar pigments, carotenoids and chlorophyll a, from Synechocystis sp. PCC 6803. For the most dose-effective concentration, pigment extraction was better with the longer alkyl-chain length, i.e., CTAB > DTAB, because the longer alkyl chain of CTAB increased cell membrane permeability, causing cell lysis that allowed solvent-pigment contact. A CTAB dose of 25 mM and treatment for 5 h gave the highest pigment extraction, nearly 30 and 12 mg/g dried biomass for chlorophyll a and carotenoids, respectively. In contrast, lipid recovery was better with the shorter alkyl-chain length, DTAB > CTAB. DTAB disrupted the cells less, which allowed the solvent to access more lipids held in the cells. Thus, the selection of surfactant and dosing strategy depends on which products are targeted. With moderate cell disruption, a moderate dose of CTAB (10 mM) was effective for recovering pigments and lipids simultaneously.
AB - Cationic surfactants can be used to improve extraction of high-value products from microalgae, but the best way to apply cationic surfactants has not been established. We evaluated the impacts of contact time and concentration for two cationic surfactants – dodecyltrimethylammonium bromide (DTAB) and hexadecyltrimethylammonium bromide (CTAB) – on extraction of lipids and nonpolar pigments, carotenoids and chlorophyll a, from Synechocystis sp. PCC 6803. For the most dose-effective concentration, pigment extraction was better with the longer alkyl-chain length, i.e., CTAB > DTAB, because the longer alkyl chain of CTAB increased cell membrane permeability, causing cell lysis that allowed solvent-pigment contact. A CTAB dose of 25 mM and treatment for 5 h gave the highest pigment extraction, nearly 30 and 12 mg/g dried biomass for chlorophyll a and carotenoids, respectively. In contrast, lipid recovery was better with the shorter alkyl-chain length, DTAB > CTAB. DTAB disrupted the cells less, which allowed the solvent to access more lipids held in the cells. Thus, the selection of surfactant and dosing strategy depends on which products are targeted. With moderate cell disruption, a moderate dose of CTAB (10 mM) was effective for recovering pigments and lipids simultaneously.
KW - Cationic surfactants
KW - Cyanobacteria
KW - Flow cytometry
KW - Harvesting and extraction
KW - Synechocystis
UR - http://www.scopus.com/inward/record.url?scp=85051271645&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85051271645&partnerID=8YFLogxK
U2 - 10.1016/j.algal.2018.08.010
DO - 10.1016/j.algal.2018.08.010
M3 - Article
AN - SCOPUS:85051271645
SN - 2211-9264
VL - 34
SP - 250
EP - 255
JO - Algal Research
JF - Algal Research
ER -