Calcium-triggered DNA-mediated membrane fusion in synthetic cells

Yen Yu Hsu; Samuel J. Chen; Julio Bernal-Chanchavac; Bineet Sharma; Hossein Moghimianavval; Nicholas Stephanopoulos; Allen P. Liu

doi:10.1039/d3cc02204h

Calcium-triggered DNA-mediated membrane fusion in synthetic cells

Yen Yu Hsu, Samuel J. Chen, Julio Bernal-Chanchavac, Bineet Sharma, Hossein Moghimianavval, Nicholas Stephanopoulos, Allen P. Liu

Molecular Sciences, School of (SMS)

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

In cells, membrane fusion is mediated by SNARE proteins, whose activities are calcium-dependent. While several non-native membrane fusion mechanisms have been demonstrated, few can respond to external stimuli. Here, we develop a calcium-triggered DNA-mediated membrane fusion strategy where fusion is regulated using surface-bound PEG chains that are cleavable by the calcium-activated protease calpain-1.

Original language	English (US)
Pages (from-to)	8806-8809
Number of pages	4
Journal	Chemical Communications
Volume	59
Issue number	57
DOIs	https://doi.org/10.1039/d3cc02204h
State	Published - Jun 7 2023

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Catalysis
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/d3cc02204h

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abstract = "In cells, membrane fusion is mediated by SNARE proteins, whose activities are calcium-dependent. While several non-native membrane fusion mechanisms have been demonstrated, few can respond to external stimuli. Here, we develop a calcium-triggered DNA-mediated membrane fusion strategy where fusion is regulated using surface-bound PEG chains that are cleavable by the calcium-activated protease calpain-1.",

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AU - Hsu, Yen Yu

AU - Chen, Samuel J.

AU - Bernal-Chanchavac, Julio

AU - Sharma, Bineet

AU - Moghimianavval, Hossein

AU - Stephanopoulos, Nicholas

AU - Liu, Allen P.

PY - 2023/6/7

Y1 - 2023/6/7

N2 - In cells, membrane fusion is mediated by SNARE proteins, whose activities are calcium-dependent. While several non-native membrane fusion mechanisms have been demonstrated, few can respond to external stimuli. Here, we develop a calcium-triggered DNA-mediated membrane fusion strategy where fusion is regulated using surface-bound PEG chains that are cleavable by the calcium-activated protease calpain-1.

AB - In cells, membrane fusion is mediated by SNARE proteins, whose activities are calcium-dependent. While several non-native membrane fusion mechanisms have been demonstrated, few can respond to external stimuli. Here, we develop a calcium-triggered DNA-mediated membrane fusion strategy where fusion is regulated using surface-bound PEG chains that are cleavable by the calcium-activated protease calpain-1.

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JO - Chemical Communications

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Calcium-triggered DNA-mediated membrane fusion in synthetic cells

Abstract

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