Ca++ and pancreatic amylase release

J. A. Williams, D. Chandler

Research output: Chapter in Book/Report/Conference proceedingChapter

34 Scopus citations


The effects of Ca++ on release of amylase by mouse pancreas in vitro were studied. The cholinergic agonist bethanechol increased release of amylase in a parallel manner in both normal and O Ca++ medium; however, release at all concentrations of bethanechol was depressed about 50% in O Ca++ medium. When pancreatic fragments were superfused with O Ca++ medium, bethanechol still produced a normal stimulation of amylase release after half of the total tissue Ca++ was washed out. This Ca++ loss included both extracellular and some intracellular Ca++. Uptake and equilibration of 45Ca++ into pancreatic fragments was multiphasic, with total equilibration with stable Ca++ still not reached after 3 hr. Addition of bethanechol had no effect on the rate of 45Ca uptake and equilibration. 45Ca++ efflux was not influenced by superfusion in O Ca++ medium, while the stimulation of 45Ca++ efflux by bethanechol was enhanced. It is concluded that extracellular Ca++ and/or Ca++ influx is not of major importance in triggering pancreatic enzyme release, but that extracellular Ca++ may regulate the release process thus accounting for the parallel changes in unstimulated and stimulated amylase release.

Original languageEnglish (US)
Title of host publicationAmerican Journal of Physiology
Number of pages4
StatePublished - 1975
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)


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