Biological diversity among serotype 2 Marek's disease viruses.

R. L. Witter, L. F. Lee, J. M. Sharma

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Selected biological characteristics were determined for 14 low-passage serotype 2 Marek's disease virus (MDV) isolates. Four of these isolates were also tested after extensive serial passage in chicken embryo fibroblast cultures. Observations were made on replication in vitro and in vivo, pathogenicity by in ovo inoculation, antigenicity, and protection against virulent MDV challenge. Among the low-passage isolates, there were some differences in pathogenicity after in ovo inoculation but relatively little difference in other characteristics, with the exception of the HN-1 strain, which replicated more rapidly in cell culture but produced generally lower in vivo responses than other isolates. After extended in vitro passage, isolates replicated much more readily in cell culture and produced lower pathologic responses in vivo than low-passage isolates, as has been reported for serotype 1 isolates. No antigenic differences among isolates were detected, but high-passage isolates induced lower levels of precipitating antibodies than low-passage isolates, indicating a possible reduction in A antigen production. The observed diversity associated with strain and passage level may be of value in the selection of optimum vaccine strains.

Original languageEnglish (US)
Pages (from-to)944-957
Number of pages14
JournalAvian diseases
Issue number4
StatePublished - 1990
Externally publishedYes

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology
  • General Immunology and Microbiology


Dive into the research topics of 'Biological diversity among serotype 2 Marek's disease viruses.'. Together they form a unique fingerprint.

Cite this