Abstract
The integration of proteins with DNA nanotechnology would enable materials with diverse applications in biology, medicine, and engineering. Here, we describe a method for the incorporation of bioactive fibronectin domain proteins with DNA nanostructures using two orthogonal coiled-coil peptides. One peptide from each coiled-coil pair is attached to a DNA origami cuboid in a multivalent fashion by attaching the peptides to DNA handles. These structures can then be assembled into one-dimensional arrays through the addition of a fibronectin domain linker genetically fused with the complementary peptides to those on the origami. We validate array formation using two different self-assembly protocols and characterize the fibers by atomic force and electron microscopy. Finally, we demonstrate that surfaces coated with the protein-DNA nanofibers can serve as biomaterial substrates for fibroblast adhesion and spreading with the nanofibers showing enhanced bioactivity compared to that of the monomeric protein.
Original language | English (US) |
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Journal | ACS Applied Bio Materials |
DOIs | |
State | Accepted/In press - 2022 |
Keywords
- DNA nanotechnology
- biomaterials
- bionanotechnology
- coiled-coils
- fibronectin
- self-assembly
- supramolecular chemistry
ASJC Scopus subject areas
- Biomaterials
- General Chemistry
- Biomedical Engineering
- Biochemistry, medical