Bioactive DNA-peptide nanotubes enhance the differentiation of neural stem cells into neurons

Nicholas Stephanopoulos; Ronit Freeman; Hilary A. North; Shantanu Sur; Su Ji Jeong; Faifan Tantakitti; John A. Kessler; Samuel I. Stupp

doi:10.1021/nl504079q

Bioactive DNA-peptide nanotubes enhance the differentiation of neural stem cells into neurons

Nicholas Stephanopoulos, Ronit Freeman, Hilary A. North, Shantanu Sur, Su Ji Jeong, Faifan Tantakitti, John A. Kessler, Samuel I. Stupp

Research output: Contribution to journal › Article › peer-review

110 Scopus citations

Abstract

We report the construction of DNA nanotubes covalently functionalized with the cell adhesion peptide RGDS as a bioactive substrate for neural stem cell differentiation. Alteration of the Watson-Crick base pairing program that builds the nanostructures allowed us to probe independently the effect of nanotube architecture and peptide bioactivity on stem cell differentiation. We found that both factors instruct synergistically the preferential differentiation of the cells into neurons rather than astrocytes.

Original language	English (US)
Pages (from-to)	603-609
Number of pages	7
Journal	Nano Letters
Volume	15
Issue number	1
DOIs	https://doi.org/10.1021/nl504079q
State	Published - Jan 14 2015
Externally published	Yes

Keywords

DNA nanotechnology
DNA-peptide
Self-assembly
biomaterials
extracellular matrix
neural stem cells

ASJC Scopus subject areas

Bioengineering
General Chemistry
General Materials Science
Condensed Matter Physics
Mechanical Engineering

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10.1021/nl504079q

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title = "Bioactive DNA-peptide nanotubes enhance the differentiation of neural stem cells into neurons",

abstract = "We report the construction of DNA nanotubes covalently functionalized with the cell adhesion peptide RGDS as a bioactive substrate for neural stem cell differentiation. Alteration of the Watson-Crick base pairing program that builds the nanostructures allowed us to probe independently the effect of nanotube architecture and peptide bioactivity on stem cell differentiation. We found that both factors instruct synergistically the preferential differentiation of the cells into neurons rather than astrocytes.",

keywords = "DNA nanotechnology, DNA-peptide, Self-assembly, biomaterials, extracellular matrix, neural stem cells",

author = "Nicholas Stephanopoulos and Ronit Freeman and North, {Hilary A.} and Shantanu Sur and Jeong, {Su Ji} and Faifan Tantakitti and Kessler, {John A.} and Stupp, {Samuel I.}",

note = "Publisher Copyright: {\textcopyright} 2014 American Chemical Society.",

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doi = "10.1021/nl504079q",

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T1 - Bioactive DNA-peptide nanotubes enhance the differentiation of neural stem cells into neurons

AU - Stephanopoulos, Nicholas

AU - Freeman, Ronit

AU - North, Hilary A.

AU - Sur, Shantanu

AU - Jeong, Su Ji

AU - Tantakitti, Faifan

AU - Kessler, John A.

AU - Stupp, Samuel I.

PY - 2015/1/14

Y1 - 2015/1/14

N2 - We report the construction of DNA nanotubes covalently functionalized with the cell adhesion peptide RGDS as a bioactive substrate for neural stem cell differentiation. Alteration of the Watson-Crick base pairing program that builds the nanostructures allowed us to probe independently the effect of nanotube architecture and peptide bioactivity on stem cell differentiation. We found that both factors instruct synergistically the preferential differentiation of the cells into neurons rather than astrocytes.

AB - We report the construction of DNA nanotubes covalently functionalized with the cell adhesion peptide RGDS as a bioactive substrate for neural stem cell differentiation. Alteration of the Watson-Crick base pairing program that builds the nanostructures allowed us to probe independently the effect of nanotube architecture and peptide bioactivity on stem cell differentiation. We found that both factors instruct synergistically the preferential differentiation of the cells into neurons rather than astrocytes.

KW - DNA nanotechnology

KW - DNA-peptide

KW - Self-assembly

KW - biomaterials

KW - extracellular matrix

KW - neural stem cells

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JO - Nano Letters

JF - Nano Letters

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Bioactive DNA-peptide nanotubes enhance the differentiation of neural stem cells into neurons

Abstract

Keywords

ASJC Scopus subject areas

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