TY - JOUR
T1 - Atomic force microscopy of long DNA
T2 - Imaging in air and under water
AU - Lyubchenko, Yuri
AU - Shlyakhtenko, Lyuda
AU - Harrington, Rodney
AU - Oden, Patrick
AU - Lindsay, Stuart
PY - 1993/3/15
Y1 - 1993/3/15
N2 - We have obtained striking atomic force microscopy images of the intact λ bacteriophage genome and of several λ restriction fragments both in air and under water. The DNA is unstained and the images are stable under continuous scanning for up to 30 min. Measured contour lengths of fully imaged restriction fragments and intact λ DNA are accurate to within a few percent. The key to this development is the use of a process for binding unmodified double-stranded DNA to chemically treated mica surfaces. This procedure leads to strong DNA attachment and yields high-quality images that are stable under repeated scanning, even with the sample submerged in water. This allows normal hydration conditions to be maintained during scanning and in addition leads to a general improvement of image quality. Both the lateral resolution and the contrast increase by a factor of ≈3 under water.
AB - We have obtained striking atomic force microscopy images of the intact λ bacteriophage genome and of several λ restriction fragments both in air and under water. The DNA is unstained and the images are stable under continuous scanning for up to 30 min. Measured contour lengths of fully imaged restriction fragments and intact λ DNA are accurate to within a few percent. The key to this development is the use of a process for binding unmodified double-stranded DNA to chemically treated mica surfaces. This procedure leads to strong DNA attachment and yields high-quality images that are stable under repeated scanning, even with the sample submerged in water. This allows normal hydration conditions to be maintained during scanning and in addition leads to a general improvement of image quality. Both the lateral resolution and the contrast increase by a factor of ≈3 under water.
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U2 - 10.1073/pnas.90.6.2137
DO - 10.1073/pnas.90.6.2137
M3 - Article
C2 - 8460119
AN - SCOPUS:0027502354
SN - 0027-8424
VL - 90
SP - 2137
EP - 2140
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 6
ER -