TY - JOUR
T1 - Atherosclerotic Yucatan microswine
T2 - An animalmodel with high-grade, fibrocalcific, nonfatty lesions suitable for testing catheter-based interventions
AU - Gal, Doy
AU - Rongione, Anthony J.
AU - Slovenkai, Geralyn A.
AU - DeJesus, Stephen T.
AU - Lucas, Alexandra
AU - Fields, Constance D.
AU - Isner, Jeffrey M.
N1 - Funding Information:
From the Departments of Biomedical Research and Medicine (Cardiology), St. Elizabeth's Hospital, Tufts University School of Medicine, Boston, Mass. Supported in part by grants from the National Heart, Lung, and Blood Institute (HL 32747; 40518), Bethesda, Md; the Whitaker Foundation, Camp Hill, Pa.; and the Eleanor Naylor Dana Charitable Trust, New York, N.Y. Work performed during Dr. Field's tenure as a Fellow of the American Heart Association's Massachusetts Affiliate, Needham, Mass., and Dr. Lucas's tenure as a fellow of the Alberta Heritage Foundation, Edmonton, Alberta, Canada. Received for publication July 19, 1989; accepted Oct. 2, 1989. Reprint requests: Jeffrey M. Isner, MD, St. Elizabeth's Hospital, 736 Cambridge St., Boston, MA 02135. 4/1/17176
PY - 1990/2
Y1 - 1990/2
N2 - A new breed of swine, the Yucatan microswine, that was derived from repetitive inbreeding of selected, small Yucatan swine, was investigated as an animal model of advanced vascular atherosclerosis. Nineteen animals were fed an atherogenic diet for 9.9±1.5 (mean±SEM) weeks before and 19.9±1.8 weeks after balloon endothelial denudation of all four iliac arteries. In 18 (94.7%) of the 19 microswine, angiography performed at 33 to 87 weeks of age disclosed some degree of luminal diameter narrowing: six animals (33.3%) had one-vessel, six (33.3%) had two-vessel, four (22.2%) had three-vessel, and two (11.1%) had four-vessel disease. In 38 (50%) of 76 denuded arteries, angiographically apparent luminal diameter narrowing was observed as follows: three arteries (7.9%) were narrowed less than 50%; 10 arteries (26.3%) were narrowed 50% to 75%; seven arteries (18.4%) were narrowed 76% to 99%; and 18 arteries (47.3%) were occluded. Sixty-four arteries were harvested from 16 of the 18 microswine with angiographically apparent luminal narrowing, which yielded 748 histologic sections. Maximum cross-sectional area narrowing from atherosclerotic plaque exceeded 90% in 135 (18%) of the sections examined, while 65 sections (9%) were narrowed 76% to 90% and 127 sections (17%) were narrowed 51% to 75%. Atherosclerotic plaque in these animals appeared histologically similar to the so-called "complex" lesion that is typical of human atherosclerosis, which consists predominantly of collagen with focal calcific deposits and a minor lipid component. The smaller, size and lower weight of these animals, in comparison with full-size farm pigs and "minipigs," facilitated transportation, handling, and instrumentation. These findings establish the Yucatan microswine as a useful, representative, and economical atherosclerotic animal model for the evaluation of novel interventional techniques.
AB - A new breed of swine, the Yucatan microswine, that was derived from repetitive inbreeding of selected, small Yucatan swine, was investigated as an animal model of advanced vascular atherosclerosis. Nineteen animals were fed an atherogenic diet for 9.9±1.5 (mean±SEM) weeks before and 19.9±1.8 weeks after balloon endothelial denudation of all four iliac arteries. In 18 (94.7%) of the 19 microswine, angiography performed at 33 to 87 weeks of age disclosed some degree of luminal diameter narrowing: six animals (33.3%) had one-vessel, six (33.3%) had two-vessel, four (22.2%) had three-vessel, and two (11.1%) had four-vessel disease. In 38 (50%) of 76 denuded arteries, angiographically apparent luminal diameter narrowing was observed as follows: three arteries (7.9%) were narrowed less than 50%; 10 arteries (26.3%) were narrowed 50% to 75%; seven arteries (18.4%) were narrowed 76% to 99%; and 18 arteries (47.3%) were occluded. Sixty-four arteries were harvested from 16 of the 18 microswine with angiographically apparent luminal narrowing, which yielded 748 histologic sections. Maximum cross-sectional area narrowing from atherosclerotic plaque exceeded 90% in 135 (18%) of the sections examined, while 65 sections (9%) were narrowed 76% to 90% and 127 sections (17%) were narrowed 51% to 75%. Atherosclerotic plaque in these animals appeared histologically similar to the so-called "complex" lesion that is typical of human atherosclerosis, which consists predominantly of collagen with focal calcific deposits and a minor lipid component. The smaller, size and lower weight of these animals, in comparison with full-size farm pigs and "minipigs," facilitated transportation, handling, and instrumentation. These findings establish the Yucatan microswine as a useful, representative, and economical atherosclerotic animal model for the evaluation of novel interventional techniques.
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U2 - 10.1016/S0002-8703(05)80019-5
DO - 10.1016/S0002-8703(05)80019-5
M3 - Article
C2 - 2301218
AN - SCOPUS:0025178571
SN - 0002-8703
VL - 119
SP - 291
EP - 300
JO - American Heart Journal
JF - American Heart Journal
IS - 2 PART 1
ER -