TY - JOUR
T1 - Assembly– disassembly is coupled to the ATPase cycle of tobacco Rubisco activase
AU - Serban, Andrew J.
AU - Breen, Isabella L.
AU - Bui, Hoang Q.
AU - Levitus, Marcia
AU - Wachter, Rebekka M.
N1 - Publisher Copyright:
© 2018 Serban et al.
PY - 2018/12/14
Y1 - 2018/12/14
N2 - The carbon-fixing activity of enzyme ribulose-1,5-bisphos-phate carboxylase/oxygenase (Rubisco) is regulated by Rubisco activase (Rca), a ring-forming ATPase that catalyzes inhibitor release. For higher plant Rca, the catalytic roles played by different oligomeric species have remained obscure. Here, we utilized fluorescence-correlation spectroscopy to estimate dissociation constants for the dimer–tetramer, tetramer– hexamer, hexamer–12-mer, and higher-order assembly equilibria of tobacco Rca. A comparison of oligomer composition with ATPase activity provided evidence that assemblies larger than hexamers are hydrolytically inactive. Therefore, supramolecular aggregates may serve as storage forms at low-energy charge. We observed that the tetramer accumulates only when both substrate and product nucleotides are bound. During rapid ATP turnover, about one in six active sites was occupied by ADP, and 36% of Rca was tetrameric. The steady-state catalytic rate reached a maximum between 0.5 and 2.5 M Rca. In this range, significant amounts of dimers, tetramers, and hexamers coexisted, although none could fully account for the observed activity profile. Therefore, we propose that dynamic assembly– disassembly partakes in the ATPase cycle. According to this model, the association of dimers with tetramers generates a hexamer that forms a closed ring at high ATP and magnesium levels. Upon hydrolysis and product release, the toroid breaks open and dissociates into a dimer and tetramer, which may be coupled to Rubisco remodeling. Although a variant bearing the R294V substitution assembled in much the same way, highly stabilized states could be generated by binding of a transition-state analog. A tight-binding pre-hydrolysis state appears to become more accessible in thermally labile Rcas.
AB - The carbon-fixing activity of enzyme ribulose-1,5-bisphos-phate carboxylase/oxygenase (Rubisco) is regulated by Rubisco activase (Rca), a ring-forming ATPase that catalyzes inhibitor release. For higher plant Rca, the catalytic roles played by different oligomeric species have remained obscure. Here, we utilized fluorescence-correlation spectroscopy to estimate dissociation constants for the dimer–tetramer, tetramer– hexamer, hexamer–12-mer, and higher-order assembly equilibria of tobacco Rca. A comparison of oligomer composition with ATPase activity provided evidence that assemblies larger than hexamers are hydrolytically inactive. Therefore, supramolecular aggregates may serve as storage forms at low-energy charge. We observed that the tetramer accumulates only when both substrate and product nucleotides are bound. During rapid ATP turnover, about one in six active sites was occupied by ADP, and 36% of Rca was tetrameric. The steady-state catalytic rate reached a maximum between 0.5 and 2.5 M Rca. In this range, significant amounts of dimers, tetramers, and hexamers coexisted, although none could fully account for the observed activity profile. Therefore, we propose that dynamic assembly– disassembly partakes in the ATPase cycle. According to this model, the association of dimers with tetramers generates a hexamer that forms a closed ring at high ATP and magnesium levels. Upon hydrolysis and product release, the toroid breaks open and dissociates into a dimer and tetramer, which may be coupled to Rubisco remodeling. Although a variant bearing the R294V substitution assembled in much the same way, highly stabilized states could be generated by binding of a transition-state analog. A tight-binding pre-hydrolysis state appears to become more accessible in thermally labile Rcas.
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U2 - 10.1074/jbc.RA118.005047
DO - 10.1074/jbc.RA118.005047
M3 - Article
C2 - 30352875
AN - SCOPUS:85058514028
SN - 0021-9258
VL - 293
SP - 19451
EP - 19465
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 50
ER -