An integrated system for continuous assessment of intercellular pH

Hany Arafa; Sahil Shah; Jennifer Blain Christen

doi:10.1109/BioCAS.2014.6981730

An integrated system for continuous assessment of intercellular pH

Hany Arafa, Sahil Shah, Jennifer Blain Christen

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

In this work we present a system that is capable of autonomously recording the pH of cultured cells in an incubator. The system is composed of an ion sensitive field effect transistor (ISFET), a transimpedance amplifier circuit, and a BeagleBone Black® computer which records ISFET output measurements. Cultured cells were grown directly on top of the ISFET, and the current output was converted to voltage via a transimpedance amplifier circuit. These converted voltage measurements were then recorded at user specified sampling rate. The system is completely self-contained with no need to run wires or cables from the inside of the incubator to the outside. We have demonstrated the system by observing cancer cell (human adenocarcinoma) proliferation over a 20 hour testing period.

Original language	English (US)
Title of host publication	IEEE 2014 Biomedical Circuits and Systems Conference, BioCAS 2014 - Proceedings
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	332-335
Number of pages	4
ISBN (Electronic)	9781479923465
DOIs	https://doi.org/10.1109/BioCAS.2014.6981730
State	Published - Dec 9 2014
Event	10th IEEE Biomedical Circuits and Systems Conference, BioCAS 2014 - Lausanne, Switzerland Duration: Oct 22 2014 → Oct 24 2014

Publication series

Name	IEEE 2014 Biomedical Circuits and Systems Conference, BioCAS 2014 - Proceedings

Other

Other	10th IEEE Biomedical Circuits and Systems Conference, BioCAS 2014
Country/Territory	Switzerland
City	Lausanne
Period	10/22/14 → 10/24/14

ASJC Scopus subject areas

Hardware and Architecture
Biomedical Engineering

Access to Document

10.1109/BioCAS.2014.6981730

Cite this

Arafa, H., Shah, S., & Blain Christen, J. (2014). An integrated system for continuous assessment of intercellular pH. In IEEE 2014 Biomedical Circuits and Systems Conference, BioCAS 2014 - Proceedings (pp. 332-335). Article 6981730 (IEEE 2014 Biomedical Circuits and Systems Conference, BioCAS 2014 - Proceedings). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/BioCAS.2014.6981730

An integrated system for continuous assessment of intercellular pH. / Arafa, Hany; Shah, Sahil; Blain Christen, Jennifer.
IEEE 2014 Biomedical Circuits and Systems Conference, BioCAS 2014 - Proceedings. Institute of Electrical and Electronics Engineers Inc., 2014. p. 332-335 6981730 (IEEE 2014 Biomedical Circuits and Systems Conference, BioCAS 2014 - Proceedings).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Arafa, H, Shah, S & Blain Christen, J 2014, An integrated system for continuous assessment of intercellular pH. in IEEE 2014 Biomedical Circuits and Systems Conference, BioCAS 2014 - Proceedings., 6981730, IEEE 2014 Biomedical Circuits and Systems Conference, BioCAS 2014 - Proceedings, Institute of Electrical and Electronics Engineers Inc., pp. 332-335, 10th IEEE Biomedical Circuits and Systems Conference, BioCAS 2014, Lausanne, Switzerland, 10/22/14. https://doi.org/10.1109/BioCAS.2014.6981730

Arafa H, Shah S, Blain Christen J. An integrated system for continuous assessment of intercellular pH. In IEEE 2014 Biomedical Circuits and Systems Conference, BioCAS 2014 - Proceedings. Institute of Electrical and Electronics Engineers Inc. 2014. p. 332-335. 6981730. (IEEE 2014 Biomedical Circuits and Systems Conference, BioCAS 2014 - Proceedings). doi: 10.1109/BioCAS.2014.6981730

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abstract = "In this work we present a system that is capable of autonomously recording the pH of cultured cells in an incubator. The system is composed of an ion sensitive field effect transistor (ISFET), a transimpedance amplifier circuit, and a BeagleBone Black{\textregistered} computer which records ISFET output measurements. Cultured cells were grown directly on top of the ISFET, and the current output was converted to voltage via a transimpedance amplifier circuit. These converted voltage measurements were then recorded at user specified sampling rate. The system is completely self-contained with no need to run wires or cables from the inside of the incubator to the outside. We have demonstrated the system by observing cancer cell (human adenocarcinoma) proliferation over a 20 hour testing period.",

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An integrated system for continuous assessment of intercellular pH

Abstract

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