@article{263a1d3b980c40d8a80c399e9d26384d,
title = "An advanced workflow for single-particle imaging with the limited data at an X-ray free-electron laser",
abstract = "An improved analysis for single-particle imaging (SPI) experiments, using the limited data, is presented here. Results are based on a study of bacteriophage PR772 performed at the Atomic, Molecular and Optical Science instrument at the Linac Coherent Light Source as part of the SPI initiative. Existing methods were modified to cope with the shortcomings of the experimental data: inaccessibility of information from half of the detector and a small fraction of single hits. The general SPI analysis workflow was upgraded with the expectation-maximization based classification of diffraction patterns and mode decomposition on the final virus-structure determination step. The presented processing pipeline allowed us to determine the 3D structure of bacteriophage PR772 without symmetry constraints with a spatial resolution of 6.9 nm. The obtained resolution was limited by the scattering intensity during the experiment and the relatively small number of single hits.",
keywords = "XFELs, bacteriophage PR772, single-particle imaging, three-dimensional virus reconstruction",
author = "Dameli Assalauova and Kim, {Young Yong} and Sergey Bobkov and Ruslan Khubbutdinov and Max Rose and Roberto Alvarez and Jakob Andreasson and Eugeniu Balaur and Alice Contreras and Hasan DeMirci and Luca Gelisio and Janos Hajdu and Hunter, {Mark S.} and Kurta, {Ruslan P.} and Haoyuan Li and Matthew McFadden and Reza Nazari and Peter Schwander and Anton Teslyuk and Peter Walter and Xavier, {P. Lourdu} and Yoon, {Chun Hong} and Sahba Zaare and Ilyin, {Viacheslav A.} and Kirian, {Richard A.} and Hogue, {Brenda G.} and Andrew Aquila and Vartanyants, {Ivan A.}",
note = "Funding Information: DA, YYK, SB, RK, MR, AT, VAI and IAV acknowledge support from the Helmholtz Associations Initiative Networking Fund grant No. HRSF-0002 and the Russian Science Foundation grant No. 18-41-06001. HD and PS acknowledge the US National Science Foundation (NSF) Science and Technology Center BioXFEL Award 1231306. HD acknowledges the Scientific and Technological Research Council of Turkey (TUBITAK) grant (118C270). JA acknowledges support from the project {\textquoteleft}Advanced research using high-intensity laser-produced photons and particles{\textquoteright} (CZ.02.1.01/0.0/0.0/16_019/0000789) from the European Regional Development Fund (ADONIS) and the Ministry of Education, Youth and Sports as part of targeted support from the National Program of Sustainability II. PLX acknowledges a grant from the European Research Council, {\textquoteleft}Frontiers in Attosecond X-ray Science: Imaging and Spectroscopy (AXSIS){\textquoteright}, ERC-2013-SyG 609920 (2014-2018), the Human Frontier Science Program (RGP0010/2017) and the fellowship from the Joachim Herz Stiftung. JH acknowledges the Swedish Research Council, the Knut and Alice Wallenberg Foundation, the European Research Council, the R{\"o}ntgen–{\AA}ngstr{\"o}m Cluster and the project {\textquoteleft}Structural dynamics of biomolecular systems{\textquoteright} (CZ.02.1.01/0.0/0.0/15_003/0000447) from the European Regional Development Fund. EB acknowledges support by the Australian Research Council Centre of Excellence in Advanced Molecular Imaging (CE140100011). The authors acknowledge use of the Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, where use is supported by the US Department of Energy, Office of Science, Office of Basic Energy Sciences under Contract No. DE-AC02-76SF00515. Publisher Copyright: {\textcopyright} 2020.",
year = "2020",
month = nov,
day = "1",
doi = "10.1107/S2052252520012798",
language = "English (US)",
volume = "7",
pages = "1102--1113",
journal = "IUCrJ",
issn = "2052-2525",
publisher = "International Union of Crystallography",
}