Abstract
The amperometric determination of dopamine under controlled convective conditions on an enzymatically modified carbon paste electrode was studied using potato tissue as a source of polyphenol oxidase. Enzymatic kinetic constants and substrate diffusional-resistance parameters were determined and an analysis of the most common interferents was made. It is concluded that the electrode is suitable for the quantification of dopamine with a detection limit as low as 2.5 × 10-6 M when the hydrodynamic conditions are carefully controlled. The electrode is active towards dopac, ascorbic acid and pyrocatechol but not l-tyrosine, l-dopa, homovanillic acid, adrenaline, normetanephrine and vanilmandelic acid.
Original language | English (US) |
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Pages (from-to) | 33-40 |
Number of pages | 8 |
Journal | Journal of Electroanalytical Chemistry |
Volume | 382 |
Issue number | 1-2 |
DOIs | |
State | Published - Feb 7 1995 |
Externally published | Yes |
Keywords
- Amperometry
- Carbon paste electrode
- Dopamine
- Enzyme electrode
ASJC Scopus subject areas
- Analytical Chemistry
- General Chemical Engineering
- Electrochemistry