Aminoacylation of transfer RNAs with one and two amino acids

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


The detailed synthesis of (bis)aminoacyl-pdCpAs and the corresponding singly and tandemly activated tRNAs is reported. The synthetic pathway leading to these compounds has been validated for simple amino acid residues as well as for amino acids bearing more complex side chains. Protection/deprotection strategies are described. For the bisaminoacylated tRNAs, both the synthesis of tRNAs bearing the same amino acid residue at the 2′ and 3′ positions and tRNAs bearing two different aminoacyl moieties are reported. Further, it is shown that the tandemly activated tRNAs are able to participate in protein synthesis.

Original languageEnglish (US)
Pages (from-to)87-99
Number of pages13
Issue number2
StatePublished - Feb 2008
Externally publishedYes


  • (Bis)aminoacylated pdCpAs
  • Aminoacylation reaction
  • Protein synthesis
  • Tandemly activated transfer RNAs
  • Transfer RNA misacylation

ASJC Scopus subject areas

  • Molecular Biology
  • General Biochemistry, Genetics and Molecular Biology


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