Acid-fast staining and Petroff-Hausser chamber counting of mycobacterial cells in liquid suspension

Robin Treuer; Shelley Haydel

doi:10.1002/9780471729259.mc10a06s20

Acid-fast staining and Petroff-Hausser chamber counting of mycobacterial cells in liquid suspension

Robin Treuer, Shelley Haydel

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Accurate and rapid cell counts of mycobacterial species in culture are difficult to obtain. Here, a method using modified Kinyoun acid-fast staining was adapted for use with a Petroff-Hausser sperm and bacteria cell-counting chamber by using a liquid suspension staining technique. Cell counts obtained by this method were compared to viable cell counts by agar plate counting, revealing accurate correlation.

Original language	English (US)
Article number	10A.6
Journal	Current Protocols in Microbiology
Issue number	SUPPL.20
DOIs	https://doi.org/10.1002/9780471729259.mc10a06s20
State	Published - Feb 2011

Keywords

Acid-fast staining
Hemacytometer
Microscope
Mycobacteria

ASJC Scopus subject areas

Parasitology
Microbiology
Virology

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10.1002/9780471729259.mc10a06s20

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abstract = "Accurate and rapid cell counts of mycobacterial species in culture are difficult to obtain. Here, a method using modified Kinyoun acid-fast staining was adapted for use with a Petroff-Hausser sperm and bacteria cell-counting chamber by using a liquid suspension staining technique. Cell counts obtained by this method were compared to viable cell counts by agar plate counting, revealing accurate correlation.",

keywords = "Acid-fast staining, Hemacytometer, Microscope, Mycobacteria",

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AU - Haydel, Shelley

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AB - Accurate and rapid cell counts of mycobacterial species in culture are difficult to obtain. Here, a method using modified Kinyoun acid-fast staining was adapted for use with a Petroff-Hausser sperm and bacteria cell-counting chamber by using a liquid suspension staining technique. Cell counts obtained by this method were compared to viable cell counts by agar plate counting, revealing accurate correlation.

KW - Acid-fast staining

KW - Hemacytometer

KW - Microscope

KW - Mycobacteria

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Acid-fast staining and Petroff-Hausser chamber counting of mycobacterial cells in liquid suspension

Abstract

Keywords

ASJC Scopus subject areas

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